We narrowed to 17,013 results for: Por

1. pSS1 (hLPL-FLAG)

   Plasmid

   #102447

   Purpose

   Expresses FLAG-tagged human LPL in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   Lipoprotein lipase (LPL Human)
   

   Tags

   6x His and FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-eGFP_mito

   Plasmid

   #221414

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   eGFP_mito
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK ARF6(Q67L)-CFP

   Plasmid

   #200428

   Purpose

   Mammalian expression of Q67L mutation in ARF6

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   PGK

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG876 pDisplay-pFAST

   Plasmid

   #172868

   Purpose

   expresses pFAST at the surface of mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   pFAST-PDGFR
   

   Tags

   IgK leader sequence (N terminal on insert) and My…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNLF1-C_hCOL4A5-Nluc_R1683X

   Plasmid

   #198124

   Purpose

   Express hCOL4A5_R1683X in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Tags

   Nluc

   Expression

   Mammalian

   Mutation

   R1683X

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2CG2-acta1:QFGal4-SV40pA

   Plasmid

   #155121

   Purpose

   Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   acta1:QFGal4
   

   Use

   Zebrafish expression

   Promoter

   acta1

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

   Plasmid

   #134613

   Purpose

   Mammalian expression of rat KIF5C 378-955

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5C (Kif5c Rat)
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 377 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. APEX2-SOPP3-TM

   Plasmid

   #229077

   Purpose

   Expresses APEX2-SOPP3 in the cell surface

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the cell surface (APEX2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-eGFP_KDEL

   Plasmid

   #221411

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   eGFP_KDEL
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-DIO-GRAB_Ado1.0med

    Plasmid

    #153292

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Y951F-VEGFR2-mCherry

    Plasmid

    #139607

    Purpose

    Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-beta1(617-949)-GFP

    Plasmid

    #100732

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP1B1 (AP1B1 Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN_TT 3xFLAG-KPNB1

    Plasmid

    #192301

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNB1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNB1 (KPNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAPTrap TALEN 2

    Plasmid

    #83369

    Purpose

    Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN2
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGAPTrap-TALEN1

    Plasmid

    #83368

    Purpose

    Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN1
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. hSyn-Aion-citrine

    Plasmid

    #192569

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

    Plasmid

    #170175

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX-UAP56

    Plasmid

    #157661

    Purpose

    Expresses UAP56 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Spliceosome RNA helicase DDX39B (DDX39B Human)
    

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-43

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-AktAR

    Plasmid

    #61624

    Purpose

    FRET-based biosensor for monitoring Akt activity

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR (AKT1 Human, Budding Yeast, Synthetic)
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

    Plasmid

    #90010

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C WT
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. VPS13C^halo∆1235-1748

    Plasmid

    #187297

    Purpose

    Express VPS13C truncation mutant in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    VPS13C truncation mutant (VPS13C Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CaMKII-soCoChR-GFP

    Plasmid

    #107711

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LENTICRISPR-TKT_sgRNA2

    Plasmid

    #201625

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LENTICRISPR-TKT_sgRNA1

    Plasmid

    #201624

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PB-Ef1a-RPS27A-UTR-PP7-WPRE

    Plasmid

    #198339

    Purpose

    PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    RPS27A-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 6K-loop-->R GLUT1-GFP

    Plasmid

    #200098

    Purpose

    human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 225, 229, 230, …

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 11K-cyto-->R GLUT1-GFP

    Plasmid

    #200096

    Purpose

    human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K-->R mutations of amino acids 6, 7, 225, 229,…

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDONR221_SLC39A8

    Plasmid

    #132177

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A8 (SLC39A8 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPPC011

    Plasmid

    #171143

    Purpose

    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Sp.pCas9, BBa_J23107

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge