We narrowed to 25,138 results for: crispr
-
Plasmid#138315PurposeLenti expression of mTgBFP2DepositorInsertmTAGBFP2
UseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA
Plasmid#192159PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVMutationNAAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXT128_TwinStrep-SUMO-ShCas12k
Plasmid#135524PurposeProtein expression construct for ShCast12kDepositorInsertShCas12k
TagsTwinStrep-SUMOExpressionBacterialAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0047-CMV-dPspCas13b-ADAR1DD(E1008Q)
Plasmid#103863PurposedPspCas13b-ADAR1DD(E1008Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA1-28-pA
Plasmid#55200PurposePlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdsRed2
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLCHKO_intergenic-intergenic_1
Plasmid#155077PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectivelyDepositorInsert(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpdCas9-VP64
Plasmid#115794PurposeExpresses SpdCas9-Vp64 fusion protein when packaged into AAVDepositorInsertVP64
UseAAVTags3XFLAG and dCas9-VP64ExpressionMammalianPromoterCMVAvailable sinceJan. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMBP-AaCas12b
Plasmid#113433PurposeExpresses Expresses 10xHis-MBP fused to AaCas12bDepositorInsertAaCas12b
TagsMBPExpressionBacterialPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBY1_FnCpf1cg
Plasmid#104622PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationcodon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one
Plasmid#85717PurposeExpresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNADepositorInsertsSp dCas9
telomere sgRNA
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianPromoterCMV-TO and U6Available sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p426-SNR52p-gRNA.csr-1.Y-SUP4t
Plasmid#68060PurposegRNA for csr-1 locus in N. crassaDepositorInsertgRNA for csr-1 locus
UseCRISPR; N. crassa, fungiExpressionYeastPromoterSNR52Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Eef1a-1955/-1>nls::Cas9::nls
Plasmid#59987PurposeEef1a (EF1alpha) promoter driving nls::Cas9::nlsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnls::Cas9::nls
UseCRISPRMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Eef1a (EF1alpha) -1955/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJV53-Cas12a
Plasmid#158706PurposeCRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61DepositorInsertFnCpf1
UseCRISPRExpressionBacterialPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-enAsCas12f
Plasmid#204637PurposeExpression of enAsCas12f in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertenAsCas12f
TagsNLSExpressionMammalianMutationD196K, N199K, G276R, N328G, D364RPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLCHKO_Ptbp_ex8_1
Plasmid#155081PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_Ptbp_ex8_4
Plasmid#155084PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_Ptbp_ex8_2
Plasmid#155082PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by