59,540 results

1. pLenti HRE-Luc pGK Hygro

   Plasmid

   #118706

   Purpose

   reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HRE (HIF-responsive promoter (3xHRE)-luc
   

   Use

   Lentiviral

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71294

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   GNSTM glycosylation motif, HA, Lamp2 signal pepti…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_GPHN.FingR-mKate2-IL2RGTC

   Plasmid

   #46297

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATG9A-EGFP

   Plasmid

   #210367

   Purpose

   Expresses ATG9A fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LENTICRISPR-UPP1_sgRNA1

   Plasmid

   #201634

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP‐RNASEH2A

   Plasmid

   #108700

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2A (RNASEH2A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIC162

   Plasmid

   #63070

   Purpose

   Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TARDBP_Halo_N_allele

    Plasmid

    #178131

    Purpose

    Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (TARDBP Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. drebrin-YFP

    Plasmid

    #40359

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Use

    Chick expression

    Tags

    eYFP

    Promoter

    chick B-actin promoter with CMV enhancer

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRES-H3.3-WT-FLAG-BFP

    Plasmid

    #206139

    Purpose

    Expression of a FLAG tagged human histone H3.3 gene with BFP

    Depositor

    Maria Castro

    Insert

    H3.3 (H3-3A Human)
    

    Tags

    FLAG-eBFP

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK-Drosha(UniProt)-FLAG

    Plasmid

    #164690

    Purpose

    Express Drosha-FLAG in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    Drosha (DROSHA Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-PAX3-FOXO1/FKHR

    Plasmid

    #115526

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

    Insert

    Pax3-FOXO1/FKHR (FOXO1, Pax3 Human, Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

    Plasmid

    #159128

    Purpose

    expresses KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7C-HSPA8

    Plasmid

    #177661

    Purpose

    Recombinant protein synthesis of human HSPA8

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA8 (HSPA8 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PPP1CA

    Plasmid

    #155843

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPP1CA (PPP1CA Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. WT1 KI gRNA1

    Plasmid

    #92312

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mApple-M1 Spastin

    Plasmid

    #134461

    Purpose

    M1 Spastin expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    Met to Ala change at amino acid 87

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TMED9-bio-His

    Plasmid

    #51855

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED9 (TMED9 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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