We narrowed to 9,193 results for: Pol;

1. pLV-H1-shSRGAP2A-hsynapsin-EGFP

   Plasmid

   #220798

   Purpose

   shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

   Insert

   EGFP
   

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScalps_puro_mIL-2Rβ

   Plasmid

   #59916

   Purpose

   Expresses mouse interleukin-2 receptor Beta chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor beta (Il2rb Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pX462-hPRKAA1-gRNA_A

   Plasmid

   #74374

   Purpose

   gRNA_A to knockout human AMPK alpha 1 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ScSpe1 pET-20b(+)

   Plasmid

   #117145

   Purpose

   Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

   Depositor

   Markus Ralser

   Article

   Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

   Insert

   SPE1
   

   Tags

   Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Frt-hspB7

   Plasmid

   #63098

   Purpose

   Expression of HSPB7 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb7 (HSPB7 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.Halo-Kif13B tail 442-1843

   Plasmid

   #162721

   Purpose

   Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   Kif 13B (Kif13b Mouse)
   

   Expression

   Mammalian

   Mutation

   WT with deletion of the 441 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-mClover3-KASH5-DN

   Plasmid

   #171492

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-GST-KASH5-DN

   Plasmid

   #171493

   Purpose

   T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   GST

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C3-PLK4 K41M-3xFLAG

   Plasmid

   #69838

   Purpose

   This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   K41M

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid

    #191334

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTMiniTrex-mCherry-Shark2

    Plasmid

    #233144

    Purpose

    Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark2
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Mutation

    Codon optimized for Trypanosoma cruzi

    Available Since

    March 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCR8/GW_TOPO_Cdk12_Untagged

    Plasmid

    #127176

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    None

    Promoter

    None

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSlb-N748D

    Plasmid

    #60732

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP109 pEF1a-gRNA-GFP

    Plasmid

    #100935

    Purpose

    GFP, puromycin and gRNA scaffold driven by EF1a promoter

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA scaffold
    

    Use

    Grna scaffold

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB80-hsKIF5B(1-560)-L-GFP

    Plasmid

    #193716

    Purpose

    Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-L-GFP (KIF5B Synthetic, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560);

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PVRL2-bio-His

    Plasmid

    #51824

    Purpose

    Expresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PVRL2 (NECTIN2 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaMKIV-dCT

    Plasmid

    #126422

    Purpose

    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-lox-3xSTOP-lox (JDW 869)

    Plasmid

    #224521

    Purpose

    A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LoxP-3xStop-LoxP
    

    Use

    Cre/Lox

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2021

    Plasmid

    #49128

    Purpose

    AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple302-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    DCX

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pAG27

    Plasmid

    #226742

    Purpose

    Expresses a reporter for a mitochondrial matrix protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEE050

    Plasmid

    #176807

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    601
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. Frt-V5-hspB7

    Plasmid

    #63108

    Purpose

    Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb7 (HSPB7 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid

    #89886

    Purpose

    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-NRasQ61K
    

    Use

    Unspecified

    Tags

    mCherry

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pQFlag-USP11 CS puroR

    Plasmid

    #46748

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pBactin-Halo-Tag

    Plasmid

    #196898

    Purpose

    Expression of Halo-Tag. Use as control for Halo-tag fusions

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Halo-Tag
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3 Flag MFF iso2 WT

    Plasmid

    #74389

    Purpose

    Flag tagged human MFF isoform 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pAAV-UbC-mEmerald Rab7a

    Plasmid

    #115238

    Purpose

    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab7a fused to mEmerald (RAB7A Human)
    

    Use

    AAV

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    7 Amino Acids (21 Nucleotides) between mEmerald a…

    Promoter

    Human UbC

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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