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We narrowed to 23,099 results for: crispr

Showing: 9751 - 9800 of 23099 results

  4. pSC25

    Plasmid
    #104799
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from Gmubi promoter from Gmubi promoter
    Depositor
    Nevin Young
    Article
    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.
    Insert
    Medtr2g101040
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    104799-plasmid-map-sequence-id-202702

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  5. pSC24

    Plasmid
    #104798
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter
    Depositor
    Nevin Young
    Article
    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.
    Insert
    Medtr2g101120, Medtr2g101130
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    104798-plasmid-map-sequence-id-202699

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  6. pSC22

    Plasmid
    #104796
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter
    Depositor
    Nevin Young
    Article
    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.
    Insert
    Medtr2g101120, Medtr2g101130
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    104796-plasmid-map-sequence-id-202698

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  7. pSC19

    Plasmid
    #104793
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoter
    Depositor
    Nevin Young
    Article
    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.
    Insert
    Medtr2g020630
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Jan. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    104793-plasmid-map-sequence-id-202692

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  29. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  32. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  33. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

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  35. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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  37. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

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  39. pT3TS-zSpG

    Plasmid
    #179316
    Purpose
    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Depositor
    Miguel Angel Moreno-Mateos
    Article
    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:
    Insert
    Zebrafish codon-optimized Cas9 variant SpG
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R
    Promoter
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    179316-plasmid-map-sequence-id-354720

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  40. pT3TS-zSpRY

    Plasmid
    #179317
    Purpose
    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Depositor
    Miguel Angel Moreno-Mateos
    Article
    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:
    Insert
    Zebrafish codon-optimized Cas9 variant SpRY
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…
    Promoter
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    179317-plasmid-map-sequence-id-354723

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  41. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

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  45. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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  47. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  48. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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Showing: 9751 - 9800 of 23099 results