165,187 results

1. HA-Foxo1ADA (pCMV5)

   Plasmid

   #12143

   Depositor

   Domenico Accili

   Article

   Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

   Insert

   Foxo1 ADA (Foxo1 Mouse)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Phosphorylation-deficient, also referred to as co…

   Promoter

   CMV

   Available Since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. PP7_mCherry

   Plasmid

   #61763

   Purpose

   Expresses PP7 coat protein fused to mCherry

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   PP7 coat protein fused to mCherry
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Ubc promoter

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yes-EGFP

   Plasmid

   #110497

   Purpose

   Fluorescent Yes

   Depositor

   Bernardo Mainou

   Insert

   Yes (YES1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPR v2-sgLKB1 clone1

   Plasmid

   #162125

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-mCherry-Nanobody (LaM-2)

   Plasmid

   #162276

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

   Insert

   mCherry-Nanobody (LaM-2)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

   Plasmid

   #238234

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-KS-FtoA
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHaMDRwt

   Plasmid

   #10957

   Depositor

   Michael Gottesman

   Article

   Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

   Insert

   MDR1 (ABCB1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCG-Hc∆18

   Plasmid

   #84817

   Purpose

   Measles virus hemagglutinin expressing plasmid

   Depositor

   Jakob Reiser

   Article

   Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

   Insert

   Measles virus H glycoprotein
   

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMD2-VSVGmut

    Plasmid

    #182229

    Purpose

    Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    VSVGmut
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162377

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pR26

    Plasmid

    #127372

    Purpose

    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Promoter

    tight TRE promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL_SRSF2_WT_mCherry

    Plasmid

    #84020

    Purpose

    expression of SRSF2 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag beta-1-adrenergic-receptor

    Plasmid

    #14698

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B1AR (ADRB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Promoter

    CMV immediate early promoter

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TMED2-bio-His

    Plasmid

    #51853

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED2 (TMED2 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #213481

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTfR-mScarlet-I

    Plasmid

    #112954

    Purpose

    In vivo visualization of Transferrin receptors (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Insert

    pTfR
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid

    #228469

    Purpose

    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Inserts

    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    

    Expression

    Bacterial

    Promoter

    J23106, J23108, and T7A1

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCN34e-kimA-yfp

    Plasmid

    #227638

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    kimA-yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. Calnexin-HT8-mGold

    Plasmid

    #183989

    Purpose

    Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Tags

    HaloTag8 (C61S, C262S), mGold

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-U6-gRNA-CBh-mCherry

    Plasmid

    #91947

    Purpose

    Expression of empty gRNA cassette and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. R777-E285 Hs.ROCK2

    Plasmid

    #70569

    Purpose

    Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Expression

    Mammalian

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAR18_StrepI_mFASm_H8_pET22b

    Plasmid

    #122847

    Purpose

    Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pUCsRNAP

    Plasmid

    #182746

    Purpose

    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Insert

    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    

    Use

    CRISPR

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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