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178,666 results

Showing: 5881 - 5910 of 178666 results

  2. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198397-plasmid-map-sequence-id-389535

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  6. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    178885-plasmid-map-sequence-id-354971

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  10. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Publication
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162279-plasmid-map-sequence-id-321873

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  12. pCMV-HyPer7-MEM

    Plasmid
    #136465
    Purpose
    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    Farnselation tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136465-plasmid-map-sequence-id-308714

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  14. GFP-Dcp1a

    Plasmid
    #153972
    Purpose
    Fluorescent protein fused to mRNA decapping enzyme 1a
    Depositor
    Gia Voeltz
    Publication
    Lee et al Science. 2020 Jan 31;367(6477). pii: 367
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRNA decapping enzyme 1a (DCP1A Human)
    Tags
    pAcGFP-C1
    Expression
    Mammalian
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153972-plasmid-map-sequence-id-306138

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  21. pSI-Target-AID-NG

    Plasmid
    #119861
    Purpose
    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.
    Depositor
    Osamu Nureki
    Publication
    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    Expression
    Mammalian
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119861-plasmid-map-sequence-id-232065

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  26. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid
    #104487
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7s
    Use
    AAV
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104487-plasmid-map-sequence-id-241275

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Showing: 5881 - 5910 of 178666 results