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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCri-1b

Plasmid

#61327

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MBP-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pMLS360

Plasmid

#73687

Purpose

Punc-47::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-47::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Promoter

Punc-47

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2-WPRE

Plasmid

#104110

Purpose

AAV-mediated expression of mTurquoise2 under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc13

Plasmid

#198283

Purpose

Produces PVCs lacking tail fibers

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc13

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsq

Plasmid

#109356

Purpose

GsX chimera with Gαq C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsq chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDAS12069_U6-pegRNA-mCherry

Plasmid

#177180

Purpose

pegRNA cloning plasmid with mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EYFP-WPRE

Plasmid

#104111

Purpose

AAV-mediated expression of EYFP under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGR-BBa_B0010

Plasmid

#46003

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

BBa_B0010 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFUGW-H1 empty vector

Plasmid

#25870

Purpose

3rd generation lentiviral vector

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 dUBA (1-389)

Plasmid

#210426

Purpose

Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL dUBA (1-389) (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Asef2

Plasmid

#129610

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

Asef2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKSJ331

Plasmid

#103063

Purpose

Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

Depositor

Alan Finkelstein, Karen Jakes

Article

Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

Insert

Colicin E1 and E1 immunity protein

Expression

Bacterial

Promoter

colicin promotor from ColE1

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDiGc

Plasmid

#59322

Purpose

Inducible expression of dsRed and constitutive expression of GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pPSup

Plasmid

#149416

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Expression

Plant

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Pex3-EGFP-APEX2-V5

Plasmid

#236401

Purpose

Expresses a fluorescent peroxisome-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

Pex3(fragment)-EGFP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-ADRP-APEX2-V5

Plasmid

#236405

Purpose

Expresses a fluorescent lipid droplet-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-ADRP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsz

Plasmid

#109355

Purpose

GsX chimera with Gαz C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsz chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo

Plasmid

#75088

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDD57

Plasmid

#119882

Purpose

Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_GFP+

Expression

Bacterial

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA221T7RNAP

Plasmid

#167974

Purpose

Expresses T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

T7RNAP (T7p07 Synthetic)

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Article

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueBac human APC2

Plasmid

#11425

Depositor

Marc Kirschner

Article

Yu et al Science. 1998 Feb 20. 279(5354):1219-22.

Insert

APC2 (ANAPC2 Human)

Use

Baculoviral

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
B-gTEMP/pRSET-b

Plasmid

#188446

Purpose

B-gTEMP for bacterial expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Tags

6xHis-tag

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs13

Plasmid

#109359

Purpose

GsX chimera with Gα13 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs13 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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phU1-CasRx-non-targeting (pKW82)

Plasmid

#229050

Purpose

Cloning backbone for CasRx crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID pRetrox

Plasmid

#155203

Purpose

For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

Depositor

Kyle Roux

Article

May et al Cells. 2020 Apr 25;9(5). pii: cells90510

Insert

3xHA-TurboID

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Promoter

tight TRE promotor

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs12

Plasmid

#109357

Purpose

GsX chimera with Gα12 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs12 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mito-EcSTH-LbNOX

Plasmid

#211922

Purpose

Mitochondrial localization of EcSTH-LbNOX

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D NAPA-A SV40

Plasmid

#92281

Purpose

FRET probe; extracellular membrane tethered eGFP

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Astrocyte

Use

AAV

Promoter

GfaABC1D

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Tags

HA

Expression

Mammalian

Mutation

C360A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pPGxRF3

Plasmid

#138384

Purpose

T2A-GAL4 knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLCP FLAG B55 alpha

Plasmid

#13804

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha Flag (PPP2R2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pZac2.1 hSynapsin1 NAPA-N SV40

Plasmid

#92282

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Promoter

hSynapsin1

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HK_13_Bla_Lenti_Kir2.1

Plasmid

#136632

Purpose

encodes Kir2.1 potassium channel for lentiviral transduction

Depositor

Adam Cohen

Article

McNamara et al Nat Phys. 2020 Mar;16(3):357-364. doi: 1

Insert

Kir2.1

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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We narrowed to 31,863 results for: ica