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pCMV-P-RAN-GFP1

Plasmid

#106408

Purpose

P-RAN-GFP1 (RANbody GFP1::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJH23

Plasmid

#159314

Purpose

Punc-25 GFP::SYD-2

Depositor

Mei Zhen

Article

Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

Insert

syd-2 (syd-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Punc-25

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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UAS-<FRT.STOP<Bxb1

Plasmid

#87643

Purpose

For creating drosophila transgenics expressing UAS-<FRT.STOP<Bxb1

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-LgBiT-FRB

Plasmid

#183403

Purpose

Expresses nuclear-localized LgBiT-FRB

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

LgBiT-FRB

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-200u

Plasmid

#13555

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi G162A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

G162A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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CF-Ins54p

Plasmid

#20155

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (INP54 Budding Yeast)

Expression

Mammalian

Mutation

Stop codon at position 331.

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM

Plasmid

#206206

Purpose

Constitutive expression of BsmAIM methyltransferase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MannII-N-10

Plasmid

#55309

Purpose

Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MannII

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSEVA47_Helper

Plasmid

#231420

Purpose

M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

gp1-gp11

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST17-UnaG

Plasmid

#203485

Purpose

For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

UnaG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMF19

Plasmid

#113662

Purpose

Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

Depositor

Miguel Valvano

Article

Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

Insert

wbbL

Promoter

Ptac

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAT28-proD/SaEGFP

Plasmid

#120321

Purpose

Bacterial eGFP labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

eGFP with Staphylococcus aureus codon optimization

Expression

Bacterial

Promoter

proD

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW PuroR-P2A-COX8ASig-Timer

Plasmid

#239559

Purpose

Expression and mitohcondrial localization of Timer

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Timer

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHCL151

Plasmid

#134455

Purpose

TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

linker-mScarlet-I for C-terminal fusion

Expression

Bacterial

Promoter

plac

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai2

Plasmid

#16369

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai2 (ORAI2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2-EYFP

Plasmid

#20939

Purpose

Mammalian expression of humanized ChR2 fused to EYFP

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-GA

Plasmid

#191666

Purpose

ddGFP-A-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddGFP-A

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBJ053_MTB_jGCaMP7f

Plasmid

#209280

Purpose

Ubiquitously expresses jGCaMP7f in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

jGCaMP7f

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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KW78_HART_ODC1_PuroR

Plasmid

#207401

Purpose

Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - PuroR

Depositor

Stephen Floor

Article

Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mini CMV

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Positron

Plasmid

#129253

Purpose

Express Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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HHM-iATPSnFR1.0

Plasmid

#102546

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Bacterial

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDD108

Plasmid

#119884

Purpose

Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

BbaJ23104_EYFP

Expression

Bacterial

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET-15b-UbcH5b/2419

Plasmid

#11475

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5b (UBE2D2 Human)

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-NCad-B1

Plasmid

#191667

Purpose

ddFP-B1-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddFP-B1

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSaI20

Plasmid

#8615

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI20

Expression

Bacterial

Mutation

N terminal threnine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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7TG

Plasmid

#24314

Depositor

Roel Nusse

Article

Brugmann et al Development. 2007 Sep . 134(18):3283-95.

Insert

7xTcf-eGFP

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2cpV

Plasmid

#37471

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pHLUK

Plasmid

#64181

Purpose

harbours marker genes HIS3, LEU2, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTF101.1gw1

Plasmid

#134768

Purpose

A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation

Depositor

Kan Wang

Article

et al Euphytica (2004) 136: 167.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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B2380_BTB-IDR

Plasmid

#215098

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR) in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-ITSN1

Plasmid

#129614

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

ITSN1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPSint

Plasmid

#149421

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Expression

Plant

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-PRKAA1

Plasmid

#30305

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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We narrowed to 31,863 results for: ica