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We narrowed to 18,759 results for: nls

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Showing: 7881 - 7920 of 18759 results

  1. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid
    #211799
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211799-plasmid-map-sequence-id-418073

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  5. pcDNA5-FRT/TO-mNeonGreen-BRD4

    Plasmid
    #204692
    Purpose
    This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Publication
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRD4 (BRD4 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    Aug. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    204692-plasmid-map-sequence-id-404997

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  9. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

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  16. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  17. pCMV-BFP-U6-sgRNA

    Plasmid
    #196725
    Purpose
    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196725-plasmid-map-sequence-id-388067

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  21. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid
    #197504
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197504-plasmid-map-sequence-id-386610

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  26. pCMV-PE2-tagRFP-BleoR

    Plasmid
    #192508
    Purpose
    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.
    Depositor
    Gerald Schwank
    Publication
    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-tagRFP-P2A-BleoR
    Tags
    BleoR, SV40 bpNLS, and tagRFP
    Expression
    Mammalian
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    192508-plasmid-map-sequence-id-377009

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  33. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

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  38. pv6_MBD1_MBD

    Plasmid
    #179404
    Purpose
    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD
    Depositor
    Tuncay Baubec
    Publication
    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MBD1_MBD
    Tags
    AviTag and EGFP
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    179404-plasmid-map-sequence-id-370526

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Showing: 7881 - 7920 of 18759 results