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We narrowed to 52,924 results for: plasmid

Showing: 9851 - 9900 of 52924 results

  1. pGBW-m4136457

    Plasmid
    #149537
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149537-plasmid-map-sequence-id-314686

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  2. pGBW-m4046858

    Plasmid
    #145658
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145658-plasmid-map-sequence-id-287835

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  3. pGBW-m4046730

    Plasmid
    #145659
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145659-plasmid-map-sequence-id-287836

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  4. pGBW-m4046823

    Plasmid
    #145634
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145634-plasmid-map-sequence-id-287811

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  5. pGBW-m4046816

    Plasmid
    #145622
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145622-plasmid-map-sequence-id-287799

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  6. pGBW-m4046791

    Plasmid
    #145586
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145586-plasmid-map-sequence-id-287763

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  7. pGBW-m4046774

    Plasmid
    #145574
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145574-plasmid-map-sequence-id-287751

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  8. pGBW-m4046846

    Plasmid
    #145575
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145575-plasmid-map-sequence-id-287752

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  9. pSB1C3-FeGFP

    Plasmid
    #138261
    Purpose
    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Insert
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138261-plasmid-map-sequence-id-273630

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  12. pFOXA3-donor

    Plasmid
    #112321
    Purpose
    CRISPR donor plasmid to tag human transcription factor FOXA3 with GFP
    Depositor
    Kevin White
    Insert
    FOXA3 homology arms flanking EGFP-IRES-Neo cassette (FOXA3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:45872401:G>T, hg38:19:45872421:C>T,…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112321-plasmid-map-sequence-id-228532

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  13. GTU-ir

    Plasmid
    #81097
    Purpose
    Gene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81097-plasmid-map-sequence-id-154264

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  14. GTL-c

    Plasmid
    #81098
    Purpose
    Gene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81098-plasmid-map-sequence-id-164508

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  15. GTT-c

    Plasmid
    #81108
    Purpose
    Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81108-plasmid-map-sequence-id-164588

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  16. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  17. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  18. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    81029-plasmid-map-sequence-id-300392

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  19. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

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  20. pcDNA-Dlx6

    Plasmid
    #99480
    Purpose
    Mammalian expression of the Distal-less Homeobox 6 gene
    Depositor
    Jhumku Kohtz
    Insert
    Dlx6 (Dlx6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    99480-plasmid-map-sequence-id-190484

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  21. LZRS-Mek1-wt1 (wt1)

    Plasmid
    #21196
    Depositor
    Paul Khavari
    Insert
    Mek1 (MAP2K1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  22. Pet28a_Human_H3.1

    Plasmid
    #42631
    Depositor
    Joe Landry
    Insert
    Human H3.1 (H3C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42631-plasmid-map-sequence-id-57746

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  23. pFlag-CMV1-hMD2

    Plasmid
    #13028
    Depositor
    Doug Golenbock
    Insert
    MD2 (LY96 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13028-plasmid-map-sequence-id-169271

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  24. Creb1

    Plasmid
    #154942
    Purpose
    Expresses Creb1 in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    Creb1 (Creb1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154942-plasmid-map-sequence-id-308575

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  25. 8522-E06

    Plasmid
    #234291
    Purpose
    Gateway ORF Entry clone of human Tgfbr1 with stop codon (for native or N-terminal fusions); T204D mutation
    Depositor
    Dominic Esposito
    Insert
    Tgfbr1 T204D (Tgfbr1 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T204D
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234291-plasmid-map-sequence-id-468906

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  26. pAWO-SUMO-3C_14M_zTDP2

    Plasmid
    #200512
    Purpose
    Expression of zebrafish TDP2 recombinant protein
    Depositor
    Antony Oliver
    Insert
    TDP2 (tdp2b Zebrafish)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200512-plasmid-map-sequence-id-396440

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  27. plenti guide Thy1.1

    Plasmid
    #128063
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128063-plasmid-map-sequence-id-255154

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  28. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid
    #177308
    Purpose
    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177308-plasmid-map-sequence-id-348855

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  30. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161815-plasmid-map-sequence-id-320654

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  31. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

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  34. pBabe-CD44v4

    Plasmid
    #107969
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v4 (Cd44 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107969-plasmid-map-sequence-id-222222

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  35. pcDNA3 humanTRAF6 (1090)

    Plasmid
    #66930
    Purpose
    Mammalian Expression of TRAF6
    Depositor
    Michael Karin
    Insert
    TRAF6 (TRAF6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66930-plasmid-map-sequence-id-190221

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  37. pCMV6-XL4 FLAG-SFRP2-Fc

    Plasmid
    #115772
    Purpose
    Expression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    SFRP2 (SFRP2 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115772-plasmid-map-sequence-id-223829

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  38. g5s pD42

    Plasmid
    #117452
    Purpose
    Expression of Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Use
    Tags
    V5-His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117452-plasmid-map-sequence-id-226811

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  39. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

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  41. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Use
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    12392-plasmid-map-sequence-id-274275

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  42. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rpsJ
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  43. PCDH-METTL16-3*Flag

    Plasmid
    #196207
    Purpose
    overexpress METTL16
    Depositor
    Jianjun Chen
    Insert
    WT METTL16 (METTL16 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NO
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196207-plasmid-map-sequence-id-383845

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  44. pfmh-hCry1

    Plasmid
    #25843
    Depositor
    Aziz Sancar
    Insert
    human Cryptochrome 1 (CRY1 Human)
    Use
    Tags
    flag, his, and myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  45. pCMV5 TBRI-His

    Plasmid
    #19161
    Depositor
    Joan Massague
    Insert
    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    19161-plasmid-map-sequence-id-364368

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  46. mSirt1-GFP

    Plasmid
    #221511
    Purpose
    Fluorescently tagged mouse Sirt1-GFP
    Depositor
    Alessandro Ori
    Insert
    Sirt1 (Sirt1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221511-plasmid-map-sequence-id-439387

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  48. pDS-Red-N1 GABAA beta3

    Plasmid
    #109143
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.
    Depositor
    Stefano Vicini
    Insert
    GABAa receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    beta3 subunit of the GABAa receptor
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109143-plasmid-map-sequence-id-210126

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  49. pcDNA3.1-Opa1

    Plasmid
    #191963
    Purpose
    Expression of OPA1.
    Depositor
    Luca Scorrano
    Insert
    Opa1 (Opa1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191963-plasmid-map-sequence-id-379657

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  50. pMT3 p38

    Plasmid
    #12658
    Depositor
    John Kyriakis
    Insert
    p38alpha (MAPK14 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 21, 2006
    Availability
    Academic Institutions and Nonprofits only
    12658-plasmid-map-sequence-id-169265

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Showing: 9851 - 9900 of 52924 results