We narrowed to 13,509 results for: GAN

1. pCDNA3 V5 mDispatched WT 2x HA

   Plasmid

   #126410

   Purpose

   Expression of V5 Dispatched WT HA in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   m-Dispatched1 (Disp1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   V5 iserted between A106 and A107

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

   Plasmid

   #174890

   Purpose

   Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   U25C PylT

   Promoter

   EF1

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR_L1-YFP-miniTurbo-L2

   Plasmid

   #127352

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and YFP

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-mScarlet

   Plasmid

   #131000

   Purpose

   AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   mScarlet
   

   Use

   AAV

   Promoter

   CaMKIIa

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SLC39A14-GFP

   Plasmid

   #104380

   Purpose

   Expresses SLC39A14/ZIP14 wild-type in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

   Insert

   SLC39A14 (SLC39A14 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p026 pCS2-EnR

   Plasmid

   #11028

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   engrailed (en Fly)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Mutation

   Engrailed repressor domain (bases encoding amino …

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. mKate2-P2A-APEX2-TAPP1-PH

   Plasmid

   #67662

   Purpose

   Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-TAPP1-PH
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR_L1-Turbo-YFP-NLS-STOP-L2

   Plasmid

   #127361

   Purpose

   gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_SC_4.26_STARR-seq

   Plasmid

   #220220

   Purpose

   AAV-STARR-seq screening vector with 4.26 promote

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   4.26

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mApple-6xHp

    Plasmid

    #214410

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

    Plasmid

    #32475

    Purpose

    Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAMQ79G,Q139G-5HT3HC
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Promoter

    Synapsin

    Available Since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_GNAQ_G-alpha

    Plasmid

    #109809

    Purpose

    Protein expression and purification of GNAQ_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAQ_G-alpha (GNAQ Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-FAB(ER-LD)

    Plasmid

    #214420

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CFAST-Halotag-6xHp

    Plasmid

    #214416

    Purpose

    Lipid droplet localization of CFAST fused to Halotag-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    deletion
    

    Tags

    CFAST-Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid

    #107939

    Purpose

    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    mCherry(dpiRNA)::ANI-1(AHPH)
    

    Expression

    Worm

    Promoter

    pie-1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHIV-EF1a-2A-ZsGreen

    Plasmid

    #231985

    Purpose

    Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    2A-ZsGreen
    

    Use

    Lentiviral

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LVDP-CArG-RE-GPR

    Plasmid

    #89762

    Purpose

    Quantitative measurement of CArG response element activity.

    Depositor

    Stelios Andreadis

    Article

    Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

    Insert

    6X CArG-RE
    

    Use

    Lentiviral

    Tags

    CMV-ZsGreen

    Expression

    Mammalian

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid

    #200905

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (pTarget NGS)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-mir-17-92

    Plasmid

    #64100

    Purpose

    expresses mir-17-92

    Depositor

    Lin He

    Article

    Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

    Insert

    miR-17-92 (MIR17HG Human)
    

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SELP-bio-His

    Plasmid

    #51639

    Purpose

    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SELP (SELP Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid

    #127351

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

    Plasmid

    #233549

    Purpose

    A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CD58-bio-His

    Plasmid

    #53153

    Purpose

    Expresses full-length CD58 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD58 (CD58 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SERPINC1-bio-His

    Plasmid

    #52076

    Purpose

    Expresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINC1 (SERPINC1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-NPC1(P691S)-FLAG

    Plasmid

    #164973

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P691S

    Promoter

    EF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. VEGFR2-YFP

    Plasmid

    #108853

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Tags

    Labeled with eYFP on the C-Terminus via a 3 amino…

    Expression

    Mammalian

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75154

    Purpose

    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-4T-1 3xFlag-CREB

    Plasmid

    #82721

    Purpose

    Expresses human CREB

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    CREB (CREB1 Human)
    

    Tags

    3XFLAG and GST

    Expression

    Bacterial

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21-pelB-LaRIgG-2

    Plasmid

    #228996

    Purpose

    For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaRIgG-2
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21-pelB-LaGIgG-12

    Plasmid

    #228999

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaGIgG-12
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Neurl1Rm-Flag pcDNA3.1

    Plasmid

    #17319

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Neuralized-like (Neurl1a Mouse)
    

    Use

    RNAi

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pLVX-NPC1(F503/4A)-FLAG

    Plasmid

    #164974

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F503A and F504A

    Promoter

    EF1a

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. P2RX1-bio-His

    Plasmid

    #53359

    Purpose

    Expresses full-length P2X purinoceptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    P2RX1 (P2RX1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TetO-FUW-Rfx4

    Plasmid

    #61544

    Purpose

    Expresses Rfx4 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Rfx4 (Rfx4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pLV_PGK_LTB4R

    Plasmid

    #208087

    Purpose

    Mammalian expression of the wild-type human LTB4 receptor LTB4R.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-hAR-L26A,F27A

    Plasmid

    #89084

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF mutant (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

40. GFP-LiMETER-v1

    Plasmid

    #113933

    Purpose

    Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    LOV2 and Rit polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge