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Caenorhabditis elegans

1,859 results

Showing: 51 - 100 of 1859 results

  8. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  14. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

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  27. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159857
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159857-plasmid-map-sequence-id-318022

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  31. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159845
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159845-plasmid-map-sequence-id-317997

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  36. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    70684-plasmid-map-sequence-id-306412

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  40. pNMSB84

    Plasmid
    #199309
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199309-plasmid-map-sequence-id-404774

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  46. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Use
    Tags
    Expression
    Bacterial and Worm
    Mutation
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

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  48. pJH2685

    Plasmid
    #191372
    Purpose
    Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Tetanus Toxin Light Chain
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Punc-4
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191372-plasmid-map-sequence-id-377546

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  49. lis-1::L4440

    Plasmid
    #11336
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 51 - 100 of 1859 results