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We narrowed to 23,150 results for: Sele

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Showing: 571 - 600 of 23150 results

  1. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

    Plasmid
    #178108
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178108-plasmid-map-sequence-id-350191

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  2. ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA

    Plasmid
    #178109
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178109-plasmid-map-sequence-id-350192

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  3. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

    Plasmid
    #173209
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173209-plasmid-map-sequence-id-339524

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  4. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #173211
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173211-plasmid-map-sequence-id-339506

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  5. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid
    #173212
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173212-plasmid-map-sequence-id-339521

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  6. pY036_ATP1A1_G3

    Plasmid
    #86617
    Purpose
    Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86617-plasmid-map-sequence-id-168852

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  7. pMpGWB418

    Plasmid
    #68683
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68683-plasmid-map-sequence-id-121813

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  8. pMpGWB421

    Plasmid
    #68686
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68686-plasmid-map-sequence-id-121825

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  20. LUC05 (L/Cys/P)

    Plasmid
    #112146
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112146-plasmid-map-sequence-id-214888

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  26. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Tags
    HA-Tag
    Expression
    Mammalian
    Promoter
    T7/CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  27. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    86611-plasmid-map-sequence-id-168847

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  28. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid
    #86610
    Purpose
    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86610-plasmid-map-sequence-id-168846

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Showing: 571 - 600 of 23150 results