We narrowed to 2,360 results for: EFS

1. C-12-BE

   Plasmid

   #135040

   Purpose

   C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjCDA1L2_1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. N-8-BE

   Plasmid

   #135023

   Purpose

   C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LpCDA1L1_1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. C-1-BE

   Plasmid

   #135029

   Purpose

   C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   PmCDA1L1_4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SepOTSγ

   Plasmid

   #68284

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EF-Sep21, 1xtRNA-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. N-4-BE

   Plasmid

   #135019

   Purpose

   C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjCDA1L1_4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SepOTSε

   Plasmid

   #68286

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EF-Sep21, 1xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SepOTSθ

   Plasmid

   #68289

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 2xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SepOTSν

   Plasmid

   #68294

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EF-Sep21, 5xtRNA-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SepOTSβ

   Plasmid

   #68283

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 1xtRNA-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MCP-YPet

    Plasmid

    #215747

    Purpose

    Expresses MS2 coat protein (MCP) fused to YPet

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C-13-BE

    Plasmid

    #135041

    Purpose

    C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LpCDA1L1_4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C-7-BE

    Plasmid

    #135035

    Purpose

    C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LpCDA1L1_1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C-6-BE

    Plasmid

    #135034

    Purpose

    C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LjDA1L2_2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C-11-BE

    Plasmid

    #135039

    Purpose

    C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LpCDA1L2_2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SepOTSη

    Plasmid

    #68288

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EF-Sep21, 2xtRNA-Sep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SepOTSζ

    Plasmid

    #68287

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS/EF-Sep, 2xtRNA-Sep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SepOTSδ

    Plasmid

    #68285

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS/EF-Sep, 1xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV SOCS-3

    Plasmid

    #11486

    Depositor

    Ronald Kahn

    Article

    Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

    Insert

    SOCS-3 (Socs3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Use

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBITE-EGFRvIII-F269-OKT3

    Plasmid

    #190681

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

    Plasmid

    #31481

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce target) EF BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry contains M9S and M16L to reduce backgroun…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEFmEGFP-mDia2

    Plasmid

    #25407

    Depositor

    Arthur Alberts

    Article

    Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

    Insert

    mDia2 (Diaph3 Mouse)
    

    Use

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Encodes mDia2

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBITE-EGFRvIII-F263-OKT3

    Plasmid

    #190680

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP Centrin2 (Nigg UK185)

    Plasmid

    #41147

    Depositor

    Erich Nigg

    Article

    Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

    Insert

    Centrin-2 (CETN2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWPI-TBX2

    Plasmid

    #185526

    Purpose

    Constitutive overexpression of human TBX2

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    TBX2 (TBX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIS1-Eef25UTR-renilla

    Plasmid

    #38235

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2 5'UTR / Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Eef2 5' UTR fused to renilla ORF

    Promoter

    Endogenous Eef2

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBITE-EGFRvIII-F265-OKT3

    Plasmid

    #190679

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCSIIpuro2-H2B-iRFP

    Plasmid

    #197352

    Purpose

    Encoding nuclear-targeted iRFP713.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    iRFP with nucleus-targeting sequence
    

    Use

    Lentiviral

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LentiV_Blast

    Plasmid

    #111887

    Purpose

    Lentiviral expression of cDNA with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LentiV_Puro

    Plasmid

    #111886

    Purpose

    Lentiviral expression of cDNA with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMB34

    Plasmid

    #122245

    Purpose

    Lentivirus delivery for stable expression of LbCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Expression

    Mutation

    Promoter

    EFs

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid

    #224899

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

    Depositor

    Thomas Norman

    Article

    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLVE-eGFP

    Plasmid

    #52581

    Purpose

    Lentiviral transfer vector driving expression of eGFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Expression

    Mutation

    Promoter

    EFs

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEF6 rglut1 flag complete

    Plasmid

    #89571

    Purpose

    rglut1 flag complete

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    rglut1 flag complete (Slc2a1 Rat)
    

    Use

    Tags

    Exo Facial

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pEF.DEST51-mVenus

    Plasmid

    #154899

    Purpose

    Expresses a fluorescent protein, mVenus

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCF730

    Plasmid

    #121668

    Purpose

    EF1a-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

47. pCF708

    Plasmid

    #121685

    Purpose

    EF1a-dTEV-protease-mTagBFP2

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    TEV protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pEF6 rglut1 HA complete

    Plasmid

    #89572

    Purpose

    rglut1 HA complete

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Mol Biol Cell. 2007 Apr;18(4):1437-46. E

    Insert

    pEF6 rglut1 HA complete (Slc2a1 Rat)
    

    Use

    Tags

    V5-His A, B, C and rglut1 HA complete

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCI-syn-fRGECO1

    Plasmid

    #125243

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D323A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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