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Showing: 951 - 1000 of 17130 results

  6. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

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  11. pMSCVpuro-F-Gcn5-NLSm

    Plasmid
    #63708
    Purpose
    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells
    Depositor
    Kai Ge
    Article
    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:
    Insert
    Gcn5 (Kat2a Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    mutated K425, K427 and K438 to R (arginine)
    Promoter
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  20. pEF1-puro/hpb1

    Plasmid
    #59976
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 headpiece (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Available since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pIRESpuro3+mRFP-Utr_CH

    Plasmid
    #64358
    Purpose
    bicistronic expression of Utrophin-mRFP and Puro resistance
    Depositor
    Matthieu Piel
    Insert
    Utrophin-mRFP (UTRN Human)
    Use
    Tags
    IRES-Puro and mRFP
    Expression
    Mammalian
    Mutation
    Q236R; aa1-261 Utrophin present
    Promoter
    CMV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    64358-plasmid-map-sequence-id-196884

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  31. pBABE.puro/TO/Flag/tGFP.HuR.S221D

    Plasmid
    #110351
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110351-plasmid-map-sequence-id-254627

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  41. pBABE.puro/TO/Flag/mKO2

    Plasmid
    #110353
    Purpose
    Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    monomeric Kusabira Orange 2
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110353-plasmid-map-sequence-id-254685

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  46. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid
    #128256
    Purpose
    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.
    Depositor
    Gabor Balazsi
    Article
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Insert
    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128256-plasmid-map-sequence-id-248948

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Showing: 951 - 1000 of 17130 results