Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 17,387 results for: puro

Showing: 951 - 1000 of 17387 results

  11. pCW-ND-Cdt1-HA-Puro

    Plasmid
    #193767
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-HA (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193767-plasmid-map-sequence-id-380216

    Enlarge

  14. pBEG R2-iPuro-L3 #BGV205

    Plasmid
    #48987
    Purpose
    Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    puromycin resistance
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    NA
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

    Enlarge

  37. pMSCVpuro-F-Gcn5-NLSm

    Plasmid
    #63708
    Purpose
    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells
    Depositor
    Kai Ge
    Article
    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:
    Insert
    Gcn5 (Kat2a Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    mutated K425, K427 and K438 to R (arginine)
    Promoter
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pEF1-puro/hpb1

    Plasmid
    #59976
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 headpiece (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Available since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

    Enlarge

Showing: 951 - 1000 of 17387 results