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pcDNA3-Flag-IDH1-R132H

Plasmid

#62907

Purpose

Expresses R132 mutant IDH1 in mammalian cells

Depositor

Yue Xiong

Publication

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

IDH1 (IDH1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deleted Arg132 (R132)

Available since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Publication

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Expression

Bacterial

Promoter

J231196

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDonor_mU6

Plasmid

#69350

Purpose

sgRNA scaffold and mouse U6 promoter

Depositor

Andrea Ventura

Publication

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

mU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

mU6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ153

Plasmid

#69304

Purpose

Gateway entry vector with pco-dCas9-3X(SRDX)

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-3X(SRDX) (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

SRDX (X3) repressor fusion; NLS fusion

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-AKAR4

Plasmid

#61619

Purpose

FRET-based biosensor for monitoring PKA activity

Depositor

Jin Zhang

Publication

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AKAR4

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Publication

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV1, AAV5, and AAV Retrograde

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pAAV-EF1a-DIO-mCherry

Plasmid

#50462

Purpose

Double floxed mCherry under the control of EF1a promoter

Depositor

Bryan Roth

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

EF1a

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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ClopHensorN

Plasmid

#50758

Purpose

Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

Depositor

Colin Akerman

Publication

Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ClopHensorN

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Promoter

CAG

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Publication

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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CAG-GFP/cre

Plasmid

#49054

Purpose

Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

Depositor

Fred Gage

Publication

Tashiro et al Nat Protoc. 2006;1(6):3049-55.

Insert

GFP/Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Publication

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 Lats1 (Nigg HS189)

Plasmid

#41156

Depositor

Erich Nigg

Publication

Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

Insert

Lats1 (LATS1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Publication

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA-SADB19N

Plasmid

#32630

Depositor

Edward Callaway

Publication

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

B19N

Expression

Mammalian

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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