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MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR

Plasmid

#75135

Purpose

Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67290

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-rev-erbα

Plasmid

#186823

Purpose

Fluorescent reporter for rev-erbα expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

rev-erbα promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

rev-erbα promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

Plasmid

#184381

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Cas9, two gRNAs targeting dystrophin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

Plasmid

#184380

Purpose

The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Myod1 (Myod1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97deltaD2

Plasmid

#17229

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

changed codon 457 to stop codon

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pENTR_Hu_PTTG1(WT)

Plasmid

#136342

Purpose

Mouse PTTG1 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer

Insert

PTTG1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-smURFP-P2A-GFP

Plasmid

#197151

Purpose

Expresses the protein of smURFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

smURFP-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4-Flag-mIL-17RA/YFP.Hygro

Plasmid

#46862

Purpose

encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-YFP

Use

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Use

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Beclin-HA S-A

Plasmid

#46994

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Article

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1

Use

Tags

HA

Expression

Mammalian

Mutation

S14A

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
mEmerald-MCM5-N-22

Plasmid

#54170

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM5

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS-AMV-α1L9'S-nAchR

Plasmid

#20684

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrna1 Mouse)

Use

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Promoter

Available since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)mGAT1CFP8

Plasmid

#41670

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-mId1

Plasmid

#20963

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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Anti-Parvalbumin [L114/38R]

Plasmid

#188182

Purpose

Mammalian Expression Plasmid of anti-Parvalbumin (Rat). Derived from hybridoma L114/38

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Parvalbumin (Rattus norvegicus) recombinant mouse monoclonal antibody (Pvalb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-LC-Myosin-N-7

Plasmid

#54920

Purpose

Localization: Light Chain Myosin, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

LC-Myosin

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-BID(D59E)

Plasmid

#21133

Depositor

Junying Yuan

Article

Li et al Cell. 1998 Aug 21. 94(4):491-501.

Insert

Flag-tagged Mutant mouse BID

Use

Tags

Flage

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) TRIM12A

Plasmid

#87061

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12A (Trim12a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-B9D2-N-18

Plasmid

#54012

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

B9D2 (B9d2 Mouse)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Use

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670-2-N1

Plasmid

#197228

Purpose

Expresses the fusion protein of H2B-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pmSTpCR21

Plasmid

#35628

Purpose

Bacterial expression of mSERT

Depositor

Howard Gu

Article

Han et al BMC Pharmacol. 2006 Mar 3;6:6.

Insert

SERT (Slc6a4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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mTalin2_F3

Plasmid

#111031

Purpose

Plasmid expresses mouse talin2 FERM domain F3

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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Ncam1_0.0.0-Fc-His

Plasmid

#72086

Purpose

Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_0.0.0 (Ncam1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19 PKC alpha

Plasmid

#8429

Depositor

Frederic Mushinski

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
CD44MMA-c002

Plasmid

#175452

Purpose

Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.

Depositor

Opher Gileadi

Insert

Cd44 (Cd44 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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