159,481 results

1. pDY1622 NOLC1 sgRNA 1

   Plasmid

   #234833

   Purpose

   sgRNA 1 for NOLC1 STITCHR insertion

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   NOLC1 sgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-mCherry

   Plasmid

   #84039

   Purpose

   Staphylococcus aureus (SaCas9) conjugated with mCherry

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-CBE6b

   Plasmid

   #215820

   Purpose

   Express CBE6b (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Cj-sgRNA

   Plasmid

   #89753

   Purpose

   U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cj-SgRNA
   

   Use

   Sgrna expression under u6 promoter

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE38

   Plasmid

   #90378

   Purpose

   RELB - NFkB gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   MAF

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Con/Fon-oScarlet

   Plasmid

   #137136

   Purpose

   Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E95D

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GLUT1-GFP

   Plasmid

   #89440

   Purpose

   GLUT1 construct

   Depositor

   Jeffrey Rathmell

   Article

   Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

   Insert

   GLUT1 (Slc2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC1A4

   Plasmid

   #131962

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A4 (SLC1A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMSCV-hygro-STING

   Plasmid

   #102598

   Purpose

   Express STING for IFN-beta luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ230-RRV

    Plasmid

    #188543

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIG-hNFATc2

    Plasmid

    #74050

    Purpose

    retroviral expression plasmid for human NFATc2/C

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-LacI

    Plasmid

    #46394

    Purpose

    Overproduction of LacI

    Depositor

    Florian Hollfelder

    Article

    Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

    Insert

    LacI
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    silent T to C mutation at position 19

    Promoter

    arabinose promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. STING-V1

    Plasmid

    #124262

    Purpose

    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CXCR7-Tango

    Plasmid

    #66265

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CXCR7 (ACKR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDule2-para-aminoPhe

    Plasmid

    #85503

    Purpose

    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

    Insert

    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32T, E107T, D158P, I159L, L162A

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMax_GFP

    Plasmid

    #177825

    Purpose

    Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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