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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNA Polymerase

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTUM4

Plasmid

#230905

Purpose

Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

Depositor

Arne Skerra

Article

Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

Inserts

FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
SurA (surA E. coli)
DsbA (dsbA E. coli)
DsbC (dsbC E. coli)

Expression

Bacterial

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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CYLBL_Neo_CAGdCas9-KRAB

Plasmid

#220439

Purpose

CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

Depositor

Yin Shen

Article

Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

Insert

dCas9

Use

CRISPR and TALEN

Tags

KRAB

Expression

Mammalian

Mutation

D10A and H840A

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSIRV-AP-1-mCherry

Plasmid

#118095

Purpose

Fluorescent reporter for AP-1 activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

mCherry

Use

Retroviral

Promoter

minimal promoter with AP-1 responsive element

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFA-sfGFP

Plasmid

#162689

Purpose

pFA vector control expressing sfGFP

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

sfGFP

Tags

C terminal 6x His tag

Expression

Bacterial

Promoter

PL tet promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Neo DEST (705-1)

Plasmid

#17392

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHR-PP7-2xmCherry-CAAX

Plasmid

#74925

Purpose

Expresses PP7-2xmCherry-CAAX

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-2xmCherry-CAAX

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

Plasmid

#214926

Purpose

Expresses presynaptic non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).cpsfGFP.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Pam-FL

Plasmid

#42570

Depositor

Vijaya Ramesh

Article

Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

Insert

human MYCBP2 (MYCBP2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Pam-FL clone lacks alternatively spliced exons 52…

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pVP141

Plasmid

#222079

Purpose

Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Insert

dCas9-PmCDA1, sfGFP and sacB

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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ER-mRFP

Plasmid

#62236

Purpose

Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

Depositor

Erik Snapp

Article

Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

Insert

ER-mRFP

Tags

mRFP1

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a-mito-dsRED2

Plasmid

#174541

Purpose

mitochondria targeted dsRED expressed off of EF1a promoter

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito

Use

Lentiviral

Tags

dsRED

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-dSpCas9

Plasmid

#238946

Purpose

N-terminal fusion of HaloTag to dSpCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

HaloTag

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMV306G13+Lux

Plasmid

#26160

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pRSCqlux

Plasmid

#236109

Purpose

LuxCDABE reporter vector for Ralstonia pseudosolanacearum filamentous.

Depositor

Dehong Zheng

Article

Huang et al Phytopathol Res (2025) 7, 78. https://do

Insert

luxCDABE

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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