We narrowed to 13,921 results for: RING

1. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLD-puro-MTS-EGFP

   Plasmid

   #190270

   Purpose

   Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

   Depositor

   Christian Münch

   Article

   Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

   Insert

   MTS-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBECAb-apr

   Plasmid

   #122001

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.CAG.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209654

   Purpose

   Non-responsive control

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NPM1_mNeonGreen_Donor

    Plasmid

    #178093

    Purpose

    HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1-mNeonGreen HDR donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJMP8923

    Plasmid

    #220904

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST14-SpyCatcher

    Plasmid

    #35044

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROP_C9_Puro_PDGFRA

    Plasmid

    #183313

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Melissa Harrison, Jill Wildonger, Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVE-eGFP

    Plasmid

    #52581

    Purpose

    Lentiviral transfer vector driving expression of eGFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209730

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209725

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDIV151

    Plasmid

    #226180

    Purpose

    Expresses Cas9 in a backbone compatible with K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAP1

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmScarlet-I3_C1

    Plasmid

    #189756

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205555

    Purpose

    Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge