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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,509 results for: GAN

Showing: 8001 - 8040 of 13509 results

  1. pMOD_B2203

    Plasmid
    #91066
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91066-plasmid-map-sequence-id-181864

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  4. SORT1-bio-His

    Plasmid
    #52024
    Purpose
    Expresses full-length Sortilin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SORT1 (SORT1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52024-plasmid-map-sequence-id-80096

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  12. pMOD_B2103b

    Plasmid
    #91062
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91062-plasmid-map-sequence-id-181865

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  18. ZJOM113

    Plasmid
    #133666
    Purpose
    Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133666-plasmid-map-sequence-id-268963

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  19. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid
    #179586
    Purpose
    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)
    Depositor
    Ali Hemmati Brivanlou
    Article
    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:
    Insert
    NLS-NG-T2A-hSHH
    Use
    Piggybac
    Tags
    NeonGreen
    Promoter
    TRE
    Available Since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    179586-plasmid-map-sequence-id-354597

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  24. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

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  25. TIMP1-bio-His

    Plasmid
    #52089
    Purpose
    Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TIMP1 (TIMP1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52089-plasmid-map-sequence-id-80365

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  28. pHJ4: LexA-VHH-Caffeine

    Plasmid
    #108242
    Purpose
    LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.
    Depositor
    Jerome Bonnet
    Article
    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102
    Insert
    LexA DNA binding domain fusing with VHH-Caffeine antibody
    Use
    Synthetic Biology
    Promoter
    pLacO1
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    108242-plasmid-map-sequence-id-212131

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  29. pUBC_HA_PP7-mVenus

    Plasmid
    #111268
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mVenus
    Use
    Synthetic Biology
    Tags
    HA, SV40 NLS, and mVenus
    Expression
    Mammalian
    Mutation
    PP7: C68S, S69G [delta]70-75 (non-aggregating)
    Promoter
    hUBC
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    111268-plasmid-map-sequence-id-212603

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  30. pET15b LFn-Fla 3A

    Plasmid
    #84872
    Purpose
    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Tags
    His
    Expression
    Bacterial
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    T7
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. TIMP3-bio-His

    Plasmid
    #53418
    Purpose
    Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TIMP3 (TIMP3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53418-plasmid-map-sequence-id-83208

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  34. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127359-plasmid-map-sequence-id-246306

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  35. pDIRECT_26H

    Plasmid
    #91150
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91150-plasmid-map-sequence-id-182284

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  36. pMOD_B2312

    Plasmid
    #91070
    Purpose
    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    PvUbi1
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91070-plasmid-map-sequence-id-181878

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Showing: 8001 - 8040 of 13509 results