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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AIF 1-90 mCherry FRB

Plasmid

#67530

Purpose

For expression of mCherry fused FRB to the mitochondrial innermembrane

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Inserts

AIF a.a. 1 - 90
mCherry
FRB

Tags

AIF 1 - 90, FRB, and mCherry

Expression

Mammalian

Mutation

T->L

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple (bidirectional) GasS

Plasmid

#196055

Purpose

Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphasS-RLuc8
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pG3H-PE3max-attR1R2

Plasmid

#213049

Purpose

Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Inserts

Cas9 nickase
M-MLV Reverse Transcriptase

Use

Note: this plasmid needs helper pvs1-vir2 for rep…

Expression

Plant

Mutation

H840A, R240K, N413K,

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgATF4-1

Plasmid

#202455

Purpose

Expression of a guide RNA targeting ATF4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

ATF4 gRNA (ATF4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pX330S-2

Plasmid

#58778

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCX-HO1-2A-EGFP

Plasmid

#74672

Purpose

Mammalian expression of HO1 and EGFP

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Insert

HO1 (HMOX1 Human)

Tags

2A-EGFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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BFP-KDEL

Plasmid

#49150

Purpose

visualization of the ER

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

BiP ER signal sequence (HSPA5 Human)

Tags

KDEL and TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Halo-KDEL

Plasmid

#124316

Purpose

Expresses ER specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

KDEL Seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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piggyBAC-5xTAPE-1-BlastR

Plasmid

#175808

Purpose

Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

Depositor

Jay Shendure

Article

Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

Insert

5xTAPE-1 construct

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Article

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

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tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

Plasmid

#177203

Purpose

encoding stblHIF under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TetO7-HSP30UTR-nLucPEST-MS2(v4)

Plasmid

#199764

Purpose

Control construct of elongation reporter to quantify elongation of LacZ

Depositor

Brian Zid

Article

Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

Insert

NanolucPEST

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#42875

Purpose

A crRNA expression plasmid for targeting a specific sequence.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR-BsaI

Use

CRISPR; E.coli

Expression

Bacterial

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac1-6His-DHX9

Plasmid

#211440

Purpose

generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

Depositor

Frank Grosse, Helmut Pospiech

Article

Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

Insert

DHX9 (DHX9 Human)

Tags

6xHis

Expression

Insect

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-GCaMP6s

Plasmid

#192945

Purpose

To drive GCaMP6s under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

Insert

GCaMP6s

Use

AAV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.mCherry

Plasmid

#248069

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mCherry

Use

AAV

Mutation

S29W.A95K

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.mCherry

Plasmid

#248071

Purpose

iATPSnFR2.A95K variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mCherry

Use

AAV

Mutation

A95K

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab4a-7

Plasmid

#56440

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab4a (RAB4A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-TID-HA3-HIS3MX6

Plasmid

#227981

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pNO201

Plasmid

#249344

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridia

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Insert

group II intron and ItrA

Use

E. coli - clostridium shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pDisplay-SNAPtag

Plasmid

#205094

Purpose

Display SNAPtag on the surface of mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

SNAPtag

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLVET-IRES-GFP

Plasmid

#107139

Purpose

A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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VPS13C^Halo

Plasmid

#232864

Purpose

Expresses human VPS13C^Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

VPS13C (VPS13C Human)

Tags

internal Halo

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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hNTo1-qgRNA-pYJA5

Plasmid

#217781

Purpose

Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Insert

Quadruple sgRNA-expression cassette for nontargeting control for gene ablation

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, mouse U6, human H1, human 7SK

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Proximal Promoter-FLuc2P

Plasmid

#154259

Purpose

Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Proximal Promoter (PPARGC1A Human)

Expression

Mammalian

Promoter

Human PGC-1alpha Proximal Promoter

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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