Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

171,009 results

Showing: 8041 - 8080 of 171009 results

  7. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72641-plasmid-map-sequence-id-132790

    Enlarge

  9. pSMPUW_6.3_M1-gateway-HA

    Plasmid
    #128439
    Purpose
    lentiviral destination vector with C-terminal HA tag
    Depositor
    Lindy Barrett
    Insert
    C-terminal HA tag
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128439-plasmid-map-sequence-id-256453

    Enlarge

  10. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    51295-plasmid-map-sequence-id-339890

    Enlarge

  18. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

    Enlarge

  20. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

    Enlarge

  26. eIF2a 3

    Plasmid
    #21809
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52D
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21809-plasmid-map-sequence-id-358561

    Enlarge

  27. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105537-plasmid-map-sequence-id-202428

    Enlarge

  28. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

    Enlarge

  34. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

    Enlarge

  38. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

    Enlarge

  39. TFORF0381

    Plasmid
    #142256
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMARCA2 (SMARCA2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142256-plasmid-map-sequence-id-454954

    Enlarge

Showing: 8041 - 8080 of 171009 results