We narrowed to 15,060 results for: pET

1. CapChR1_pmCherry-N1

   Plasmid

   #188031

   Purpose

   Expresses CapChR1-mCherry in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR1-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   S63D, L132C, T159C and N258E mutations in CrChR2

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEYFP-N1-eKR2

   Plasmid

   #115337

   Purpose

   Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

   Depositor

   Peter Hegemann

   Article

   Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

   Insert

   eKR2
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO mouse shRNA 2 raptor

   Plasmid

   #21340

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

   Insert

   raptor shRNA 2 (Rptor Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pGEX NBP1 (P#415)

   Plasmid

   #14658

   Depositor

   Matija Peterlin

   Article

   Lu et al Immunity. 1998 May . 8(5):647-56.

   Insert

   ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
   

   Expression

   Bacterial

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pOPO717

   Plasmid

   #195302

   Purpose

   pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and lacZ spacer 5 in native array
   

   Expression

   Bacterial

   Promoter

   Arabinose inducible arapBAD

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-puro human lipin 1-1 shRNA

   Plasmid

   #32019

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   lipin1 shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. bPAC-mycHis_pGEM

   Plasmid

   #85468

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp.

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   Bacterial Photoactivated Adenylyl Cyclase
   

   Tags

   His and Myc

   Promoter

   T7 promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pECFP-Rac1Y40C

   Plasmid

   #105293

   Purpose

   Control for FRET biosensor analysis - cannot bind downstream effectors

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Y40C

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLEX_313 GFP

   Plasmid

   #184494

   Purpose

   Lentivirus expression for GFP

   Depositor

   Todd Golub, Peter Tsvetkov

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   GFP
   

   Use

   Lentiviral

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CapChR2_pUAST-attb

    Plasmid

    #188041

    Purpose

    Expresses CapChR2 in Drosophila

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2
    

    Tags

    none

    Expression

    Insect

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    UAS

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCytERM-hfYFP

    Plasmid

    #186520

    Purpose

    Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPO635

    Plasmid

    #195297

    Purpose

    pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABC operon of McCAST (Tn7575)
    

    Expression

    Bacterial

    Promoter

    IPTG inducible lac promoter

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSYN-VWF-039

    Plasmid

    #120292

    Purpose

    VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

    Depositor

    Robert Peters

    Article

    Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

    Insert

    VWF D1-D2-D'-D3-Fc
    

    Tags

    Fc

    Expression

    Mammalian

    Mutation

    C1099A/C1142A

    Promoter

    CMV

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPO636

    Plasmid

    #195299

    Purpose

    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABCQ operon of McCAST (Tn7575)
    

    Expression

    Bacterial

    Promoter

    IPTG inducible lac promoter

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pECFP-Cdc42-Y40C

    Plasmid

    #105295

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC110

    Plasmid

    #118905

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and LPETGg

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH

    Plasmid

    #239740

    Purpose

    Plasmid expressing a rapamycin-inducible transcriptional activator

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJB65

    Plasmid

    #226319

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (VTG to AAA MR1-6)-strep
    

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBDP

    Plasmid

    #217827

    Purpose

    Stuffer plasmid with golden gate compatible bidirectional promoter

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    Gal1/Gal10 Bidirectional Promoter
    

    Use

    Cloning vector

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSrtA-MT4

    Plasmid

    #200306

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT4 (MT4 Human)
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pnEA-NpM-Hs4EBP1_50-83-GB1_AA

    Plasmid

    #148275

    Purpose

    Bacterial Expression of Hs4EBP1_50-83

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83 (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pnEA-NpM-Hs4EBP1_50-83-L75AV81A-GB1_AA

    Plasmid

    #148276

    Purpose

    Bacterial Expression of Hs4EBP1_50-83-L75AV81A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83-L75AV81A (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pnEA-NpM-Hs4EBP1_50-83-Y54AL59A-GB1_AA

    Plasmid

    #148277

    Purpose

    Bacterial Expression of Hs4EBP1_50-83-Y54AL59A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83-Y54AL59A (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pnEA-NpM-Hs4EBP1_50-83-YLLVtoAAAA-GB1_AA

    Plasmid

    #148278

    Purpose

    Bacterial Expression of Hs4EBP1_50-83-YLLVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83-YLLVtoAAAA (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pnEA-NpM-Hs4EBP3_36-67-GB1_AA

    Plasmid

    #148279

    Purpose

    Bacterial Expression of Hs4EBP3_36-67

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP3_36-67 (EIF4EBP3 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pnYC-NpH-HseIF4E_36-217_AA

    Plasmid

    #148282

    Purpose

    Bacterial Expression of HseIF4E_36-217

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    HseIF4E_36-217 (EIF4E Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pnEA-NpM-DmCUP_325-376-R329E-GB1_AA

    Plasmid

    #148263

    Purpose

    Bacterial Expression of DmCup_325-376-R329E

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmCup_325-376-R329E (cup Fly)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pnEA-NpM-DmCUP_325-376-R329ER336E-GB1_AA

    Plasmid

    #148264

    Purpose

    Bacterial Expression of DmCup_325-376-R329ER336E

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmCup_325-376-R329ER336E (cup Fly)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pnEA-NpM-DmCUP_325-376-R329ER336ER375E-GB1_AA

    Plasmid

    #148265

    Purpose

    Bacterial Expression of DmCup_325-376-R329ER336ER375E

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmCup_325-376-R329ER336ER375E (cup Fly)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pnEA-NpM-DmCUP_325-376-R336E-GB1_AA

    Plasmid

    #148266

    Purpose

    Bacterial Expression of DmCup_325-376-R336E

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmCup_325-376-R336E (cup Fly)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge