We narrowed to 58,413 results for: tra

1. pCMV NucleoBAS N354K-I372V

   Plasmid

   #63574

   Purpose

   Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

   Depositor

   Stan van de Graaf

   Article

   van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

   Insert

   NucleoBAS N354K-I372V
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   N345K and I372V

   Promoter

   CMV

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAR-9.47_VP2_AS

   Plasmid

   #74241

   Purpose

   Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47-VP2_AS
   

   Use

   AAV

   Tags

   19 alanines fused to VP2 of AAV9.47

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUMO CPTP

   Plasmid

   #170735

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   CPTP (CPTP Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist-SARS-CoV-2 Δ18 B.1.429

   Plasmid

   #169461

   Purpose

   Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.429 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-SARS-CoV-2 Δ18 B.1.1.298

   Plasmid

   #169460

   Purpose

   Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

   Plasmid

   #58275

   Purpose

   mammalian expression of human RdgBbeta splice variant 1

   Depositor

   Shamshad Cockcroft

   Article

   Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

   Insert

   RdgBbeta (PITPNC1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-EF1a-CCre-MBP4-WPRE

   Plasmid

   #193919

   Purpose

   Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   CCre-MBP4
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-NCre-MBP5-WPRE

   Plasmid

   #193918

   Purpose

   Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   NCre-MBP5
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Δ18 P.2/B.1.1.248

   Plasmid

   #173477

   Purpose

   Encodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 Spike Truncated to remove 18 amino acids
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

    Plasmid

    #176535

    Purpose

    Non-protein coding locus components from CRISPR-associated IscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV4Neo-murine IL-17RASEFIR-HA

    Plasmid

    #46863

    Purpose

    expresses mouse IL-17RA with SEFIR deletion

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-delta-SEFIR (Il17ra Mouse)
    

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Deletion of SEFIR domain (AA 394-485)

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pPHR-mCherry-NLS-VP16

    Plasmid

    #103821

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHR-iRFP713-NLS-VP16

    Plasmid

    #103823

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

    Plasmid

    #176588

    Purpose

    Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Delaware Bay) locus
    

    Expression

    Bacterial

    Mutation

    Replaced spacer in CRISPR array with Fn spacer

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-CCre-MBP1-WPRE

    Plasmid

    #193917

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP1
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-NCre-MBP6-WPRE

    Plasmid

    #193916

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP6
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFLAG R106L-CPTP

    Plasmid

    #170744

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R106L

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLAG K60A-CPTP

    Plasmid

    #170743

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K60A

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HOXA10 (human) bluescript

    Plasmid

    #8617

    Depositor

    Corey Largman

    Article

    Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

    Insert

    HOXA10 (HOXA10 Human)
    

    Expression

    Bacterial

    Mutation

    improved Ribosomal binding site (NcoI site) at AT…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only