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pEGFP-Akt(R25C)

Plasmid

#39532

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (Akt1 Mouse)

Use

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

R25C

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB

Plasmid

#131350

Purpose

Expresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-GyrB (Myd88 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Elk1 pShuttle

Plasmid

#121097

Purpose

Expression of full length ELK1 in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSiP1

Plasmid

#21952

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2010

Availability

Academic Institutions and Nonprofits only
TrpC5 scFv [N67/15]

Plasmid

#190564

Purpose

Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC5 [N67/15R]

Plasmid

#182118

Purpose

Mammalian Expression Plasmid of anti-TrpC5 (Human). Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC5 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEF‐PIK3IP1

Plasmid

#49214

Purpose

expresses PIK3IP1 in mammalian cells

Depositor

Lawrence Kane

Article

DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

Insert

PIK3IP1 (Pik3ip1 Mouse)

Use

Tags

6X-his and myc

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf7

Plasmid

#163597

Purpose

Expresses GDF-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf7 (Gdf7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Some Guanin and Cytosine were substituted to redu…

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Pan-Ankyrin [N388A/60R]

Plasmid

#128640

Purpose

Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MCM7-N-22

Plasmid

#54174

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM7

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
HA-Nore1

Plasmid

#1979

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

Nore1 (Rassf5 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
GFP-mROCK1-delta7

Plasmid

#101295

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

727-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 Flag-Par6 delta 250-295 Flag tagged

Plasmid

#24646

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Par6c (Pard6a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the PDZ domain of Par6c (aa 250-295)

Promoter

Available since

June 2, 2010

Availability

Industry, Academic Institutions, and Nonprofits
sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Expression

Mutation

Promoter

U6, hSyn

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
tdEos-Beta-Catenin-20

Plasmid

#57586

Purpose

Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Beta-Catenin (Ctnnb1 Mouse)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRQ-Id1HA

Plasmid

#18112

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

Inhibitor of differentiation 1 (Id1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-HSV2.3 TCR beta chain

Plasmid

#74077

Purpose

gBT-I TCR beta chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta cDNA clone HSV2.3 (Tcrb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pBS/U6/RNAi SadBs shRNA mouse

Plasmid

#67152

Purpose

pBS/U6 containing shRNA to mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (Brsk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F1bbs kan GCGTGGNNN

Plasmid

#18753

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Use

Tags

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-GABARα1N-HA

Plasmid

#128110

Purpose

Express GABARα1 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra1 (Gabra1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
C-Ag15

Plasmid

#11868

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Use

Tags

His and myc

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
Anti-NPY [L115/13R]

Plasmid

#177461

Purpose

Mammalian Expression Plasmid of anti-NPY/Neuropeptide Y (Human). Derived from hybridoma L115/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NPY/Neuropeptide Y (Homo sapiens) recombinant mouse monoclonal antibody (NPY Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
CyclinB-GFP

Plasmid

#54465

Purpose

CyclinB-GFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

CyclinB

Use

Tags

GFP

Expression

Mammalian

Mutation

K67E and I150T mutations compared to GenBank refe…

Promoter

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only
pBabe JunD-Venus puro

Plasmid

#58496

Purpose

mammalian expression of Venus-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
Flag_MmPERK_S503_N1114_pBabePuro

Plasmid

#58423

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

PERK (Eif2ak3 Mouse)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids M1 to Y502

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.U6.miR-132-Decoy.hPGK.GFP.WPRE

Plasmid

#46587

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-132 Decoy (MIR132, Mir132 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only