59,540 results

1. pcDNA3/Flag-WTAP

   Plasmid

   #53741

   Purpose

   For expression of WTAP (Wilms tumor 1 associated protein) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Wilms tumor 1 associated protein (WTAP Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p663-UBC-V5-BRSK1_IDG-K

   Plasmid

   #135270

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-BRSK1 (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK-flag-USP19

   Plasmid

   #78597

   Purpose

   Express flag-USP19 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 (USP19 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MEK1C222

   Plasmid

   #164638

   Purpose

   Bacterial expression plasmid for His6-MEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S222C/C277S/C376S

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GAPDH

   Plasmid

   #155622

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GAPDH (GAPDH Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA WDR24 pRK5

   Plasmid

   #46335

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   WDR24 (WDR24 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiPGK Hygro DEST ERKKTRmCerulean3

   Plasmid

   #90229

   Purpose

   Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE2 mCherry-Rtn4a

   Plasmid

   #86683

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4a (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-VAMP8

   Plasmid

   #45912

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   vesicle-associated membrane protein 8 (VAMP8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3 HA E2F3B

    Plasmid

    #236438

    Purpose

    transient overexpression of E2F3B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3B (E2F3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Wnt3A

    Plasmid

    #35908

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt3A (WNT3A Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPR v2 hBAK

    Plasmid

    #129579

    Purpose

    CRISPR deletion of human BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs human BAK (BAK1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFUW-VenusFlag-hCD44

    Plasmid

    #211824

    Purpose

    Express CD44 in mammalian cells

    Depositor

    Haian Fu

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    N-Terminal Venus and Flag tags

    Expression

    Mammalian

    Mutation

    isoform corresponds to UniProt ID P16070-1 with a…

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Kate Fitzgerald, Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

17. EGFP-Rab7A

    Plasmid

    #28047

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RAD21 C-tag CRISPR

    Plasmid

    #140540

    Purpose

    RAD21 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HIP-flash (HOP-flash mutant)

    Plasmid

    #83466

    Purpose

    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge