We narrowed to 22,077 results for: his

1. pETSUMO-TtAgocatdead

   Plasmid

   #210322

   Purpose

   Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

   Depositor

   Phillip Zamore

   Article

   Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

   Insert

   TtAgo (TT_P0026 Thermus thermophilus)
   

   Use

   Tags

   6x His tag and SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLN351 (FuGW-S(YBX1)p-mKate2 )

   Plasmid

   #105174

   Purpose

   synthetic promoter YBX1 drives mKate2 expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(YBX1)p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLN352 (FuGW-S(Elk1)p-mKate2)

   Plasmid

   #105175

   Purpose

   synthetic promoter Elk1 drives mKate2 experssion

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(Elk1)p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pLN381 (FuGW-S(NFY)p-mKate2)

   Plasmid

   #105179

   Purpose

   synthetic promoter NFY drives mKate2 expression

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(NFY)p-mKate2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 STAT3

   Plasmid

   #67120

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pet16b ALIX Bro1 (1-359)

   Plasmid

   #80641

   Purpose

   Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   ALIX Bro1, residues 1-359 (PDCD6IP Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Residues 1-359

   Promoter

   T7

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIE055 ZF43x3-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138736

   Purpose

   Integration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x3-Spaced
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHIE052 ZF43x3-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138733

   Purpose

   Integration Vector, assembled with pHIE044 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x3-Compact
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138737

    Purpose

    Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Spaced
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138738

    Purpose

    Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Spaced
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138735

    Purpose

    Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GTH-y

    Plasmid

    #81105

    Purpose

    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCitrine
    

    Use

    Cre/Lox

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Mutated mCitrine for monomeric isoform (A206K L22…

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid

    #107421

    Purpose

    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    mutation of glutamate 77 to glutamine

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLN431 (FuGW-G8p-mKate2-1Pe )

    Plasmid

    #105186

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-mKate2-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Native

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM33_OXSR1_CCT

    Plasmid

    #178482

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    OXSR1_OXSR1 (OXSR1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p5B3-T

    Plasmid

    #92021

    Purpose

    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hNedd4-2 CS

    Plasmid

    #86041

    Purpose

    full length NeddL3 with C to S mutation with His Xpress tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

    Insert

    hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    C942S mutation in HECT domain

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLN481 (FuGW-G8p-IL12-1Pe)

    Plasmid

    #105191

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-IL12-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSKB3-SaGGR-I206F-L377H (JBEI-7202)

    Plasmid

    #110152

    Purpose

    pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

    Depositor

    Jay Keasling

    Article

    Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

    Insert

    GGR
    

    Use

    Tags

    TEV-cleavable His-tag

    Expression

    Bacterial

    Mutation

    SaGGR-I206F-L377H mutant gene

    Promoter

    T7

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDB368

    Plasmid

    #110276

    Purpose

    gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Insert

    gHH_44
    

    Use

    Tags

    10-His, Tev recognition peptide, and mature DsbC …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p5A1-Ti

    Plasmid

    #92018

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V, C151A

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLN491 (FuGW-G8p-CCL21-1Pe)

    Plasmid

    #105193

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-CCL21-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p5A3-T

    Plasmid

    #92019

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Syn3-27-146+TM

    Plasmid

    #14679

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 27-14…

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

27. Syn3D38

    Plasmid

    #13397

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3D38 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. First N-…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pET24a_SMOX

    Plasmid

    #186345

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-THTAP-SPARTIN, residues 1-101

    Plasmid

    #180626

    Purpose

    Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPARTIN
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-101

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCA528 USP54, residues 731-878

    Plasmid

    #180637

    Purpose

    Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    USP54 (USP54 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge