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Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PRKRA
Plasmid#31571DepositorInsertprotein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-ING2
Plasmid#13295DepositorInsertING2 (INGX Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 SWS cone opsin
Plasmid#109363PurposeHuman SWS cone opsin with 1D4 tagDepositorInsertSWS cone opsin (OPN1SW Human)
UseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC-SmBiT
Plasmid#207632PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiTDepositorInsertphotocaged SpyCatcher-SmBiT
UseTags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pB80-2xFKBP-L-mCherry-CAAX
Plasmid#193713PurposeRapalog-inducible coupling to plasma membrane (CAAX) via FKBPDepositorInsert2xFKBP-L-mCherry-CAAX
UseTags2xFKBP-mCherryExpressionMammalianMutationmCherry: Met1 DeletedPromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046731
Plasmid#145633PurposeBacterial Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;ā¦Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;ā¦Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDV05.1_pEF1a-iRFP720_frameshifted
Plasmid#200408PurposeExpresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent proteinDepositorInsertiRFP720 (frameshift mutant)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Blast NT shRNA
Plasmid#123306PurposeExpression of a non-targeting shRNADepositorInsertNon-targeting shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF0
Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_KanR neo
Plasmid#167861PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-ySet2
Plasmid#51698PurposeExpresses full length yeast Set 2 gene in a mamalian expression vectorDepositorInsertyeast Set2 (SET2 Budding Yeast)
UseTagsC-terminal FLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;ā¦Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pEGFP-N1-mt-ro2GFP
Plasmid#82408PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cellsDepositorInsertro2GFP
UseTagsleader sequence of the E1α subunit of pyruvate deā¦ExpressionMammalianMutationC48S/F64L/S147C/Q204CPromoterCMVAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12RRFuzzy3MYB
Plasmid#194441PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, RR, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
UseTagsExpressionBacterialMutationwild typePromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-T43NTR-TrxA-H10
Plasmid#121677PurposeBacterial expression of neurotensin receptor fusion proteinDepositorInsertneurotensin receptor (Ntsr1 Rat)
UseTagsTrxA-H10ExpressionBacterialMutationdeleted amino acids 1-42PromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ING2
Plasmid#13294DepositorInsertING2 (INGX Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STMN1
Plasmid#31326DepositorInsertstathmin 1/oncoprotein 18 (STMN1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK1
Plasmid#22896DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MCM3-N-22
Plasmid#54166PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertMCM3
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4531
Plasmid#132366PurposePrgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.DepositorInsertDAF-2 (daf-2 Nematode)
UseTagsnoneExpressionWormMutationPromoterPrgef-1Available sinceSept. 22, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
px330 p300 gRNA
Plasmid#165591PurposeInsertion of p300 degronDepositorInsertP300 (Smarca2 Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_CSF1R
Plasmid#183277PurposeAll-in-One CRISPRko system with a guide RNA that targets CSF1R geneDepositorInsertCSF1R
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;ā¦Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
L40C-CRISPR.EFS.dTomato
Plasmid#89392PurposeLentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter drivenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21c-IF3mt
Plasmid#31078DepositorInsertMature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Amiā¦PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEBB-II-I-GFP alpha isoform
Plasmid#22146DepositorInsertTFII-I (GTF2I Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJH3185
Plasmid#132367PurposePrgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFPDepositorInsertDAF-16a (daf-16 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrgef-1Available sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH4
Plasmid#52529Purposeserves as PCR-template to generate HR donors for C-terminal 2xFLAG-tagDepositorInsert2x FLAG-tag
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p6609 MSCV-IP N-HAonly PTENP1
Plasmid#35008DepositorInsertPTENP1 (PTENP1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-SOS
Plasmid#208279PurposeExpresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSW FLEx mCherry-KASH
Plasmid#139652PurposeConditional expression of mCherry-KASHDepositorInsertmCherry-KASH
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA4 (MSW#517)
Plasmid#208070PurposeExpression of GFP-tagged hsRPA4 in human cellsDepositorInsertRPA4 (RPA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP/WT SP M85
Plasmid#89317PurposeExpression of mouse GFP/WT spastin M85 in mammalian cellsDepositorInsertSPAST M85 (Spast Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only