We narrowed to 34,402 results for: CaS

1. pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

   Plasmid

   #131355

   Purpose

   Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Caspase-1 (C285A)

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

   Plasmid

   #68337

   Purpose

   Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

   Depositor

   Feng Zhang

   Insert

   St3Cas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDF0044 CjcCas7-11 full locus with synthetic array

   Plasmid

   #172502

   Purpose

   Encodes the CjcCas7-11 full locus for bacterial expression

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   CjcCas7-11 full locus with synthetic array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

   Plasmid

   #114089

   Purpose

   CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized FnCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

   Plasmid

   #135979

   Purpose

   gRNA against the left loxP site of the Ai9 allele

   Depositor

   William Lagor

   Insert

   Ai9 gRNA1
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   HA

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

   Plasmid

   #117411

   Purpose

   AsCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   AsCas12a gRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDF0039 DisCas7-11 only expression with CRISPR array

   Plasmid

   #172500

   Purpose

   Encodes the DisCas7-11 protein only for bacterial expression

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 only expression with CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

    Plasmid

    #209781

    Purpose

    Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    cTNT
    

    Use

    AAV

    Tags

    HA and OLLAS

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

    Plasmid

    #209782

    Purpose

    Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    sgRNA
    

    Use

    AAV

    Tags

    HA and OLLAS

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid

    #134331

    Purpose

    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized Nme2Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid

    #101176

    Purpose

    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K848A/K1003A/R1060A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid

    #115142

    Purpose

    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Mb3Cas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

    Plasmid

    #91880

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

    Plasmid

    #71213

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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