We narrowed to 7,456 results for: pac

1. pFUGW-RapACR-EYFP

   Plasmid

   #111075

   Purpose

   Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-EsaR-D91G

   Plasmid

   #47646

   Purpose

   pAC-EsaR with a a272g mutation in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-D91G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 91 to Glycine

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pAC-EsaR-EsaI

   Plasmid

   #47660

   Purpose

   esaI gene downstream of esaR in pAC-EsaR (CmR)

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   esaI-term
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAc-GFP-dSUMO

   Plasmid

   #85696

   Purpose

   expression of GFP-SUMO1 in Drosophila cells

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   SUMO (Sumo Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pAC1814_pCR8-gSMN2-DN2

   Plasmid

   #118647

   Purpose

   Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-DN2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC155-pCR8-sgExpression

   Plasmid

   #49045

   Purpose

   U6-driven sgRNA expressing cassette on a gateway donor vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC1815_pCR8-gSMN2-DN3

   Plasmid

   #118648

   Purpose

   Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-DN3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC1803_pmax-RBM38-dCasRx

   Plasmid

   #118637

   Purpose

   Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBM38-dCasRx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid

    #197566

    Purpose

    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    N166A, V168G, W239C

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAct-Fok1:dCas9

    Plasmid

    #62211

    Purpose

    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Spike Display_Part 2 Spacer

    Plasmid

    #172730

    Purpose

    Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. INCTbiosyn-pAct-AtINTphiC31

    Plasmid

    #127535

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase phiC31.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase phiC31 coding sequence codon optimized for A. thaliana expression.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-DmGIGYF_AH

    Plasmid

    #148918

    Purpose

    Insect Expression of DmGIGYF

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-EGFP-DmAGO1

    Plasmid

    #22768

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    Argonaut-1, variant A (AGO1 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD554 ZF129x6-Compact_EYFP

    Plasmid

    #138868

    Purpose

    EYFP reporter vector for the ZF129x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF129x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF129x6-Compact

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid

    #146978

    Purpose

    Insect Expression of DmARC1

    Depositor

    Elisa Izaurralde

    Insert

    DmARC1 (Arc1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    5 silent mutations and one non silent mutation G3…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC1807_pmax-dCasRx-FKBP

    Plasmid

    #118640

    Purpose

    Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx-FKBP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACYC_HA-ZmSAE1_ZmSAE2-FLAG

    Plasmid

    #91944

    Purpose

    Bacterial expression of maize SAE1 and SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Use

    Tags

    FLAG and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. INCTbiosyn-pAct-AtINT13

    Plasmid

    #127534

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase 13.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 13 coding sequence codon optimized for A. thaliana expression.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAC1808_pmax-RBFOX1N-FRB-C

    Plasmid

    #118641

    Purpose

    Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-FRB-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHSP70B-CP6-pac

    Plasmid

    #165877

    Purpose

    Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pACYC-Cas12g1-noncoding

    Plasmid

    #120880

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12g1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. T6-pAC5.1 mEGFP-EcoRV

    Plasmid

    #206489

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pF143 pAcGFP1 SOD1G37R

    Plasmid

    #26404

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G37R

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Expression

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. INCTbiosyn-pAct-AtINTBxb1

    Plasmid

    #127536

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. IL2 F42A pAcGP67a

    Plasmid

    #41807

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Phenylalanine 42 to Alanine

    Promoter

    Polyhedron

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pAC-EsaR-V220A

    Plasmid

    #47645

    Purpose

    pAC-EsaR with a c659t mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-V220A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Valine 220 to Alanine

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only