2,226 results

1. pcDNA TrkB

   Plasmid

   #24088

   Depositor

   Moses Chao

   Article

   Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

   Insert

   TrkB (Ntrk2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R133I

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. rGluA1 (px330:2-guideCas9)

   Plasmid

   #169437

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HSV-DYN-hM4Di

   Plasmid

   #53327

   Purpose

   Dynorphin promoter driving hM4Di

   Depositor

   John Neumaier

   Article

   Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

   Insert

   hM4Di (Pdyn Rat)
   

   Use

   Hsv

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Rat dynorphin promoter ( -1858 to +135)

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP-PKCbetaII-YFP

   Plasmid

   #14866

   Depositor

   Alexandra Newton

   Article

   Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

   Insert

   YFP-PKCbetaII-YFP
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

5. Dyn1(K44A)-mRFP

   Plasmid

   #55795

   Purpose

   Dominant negative Dynamin 1 mutant tagged with mRFP

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Dynamin-1(K44A)-mRFP (Dnm1 Rat, Discosoma sp.)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…

   Promoter

   CMV

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE mEos3.2-Camk2a KI

   Plasmid

   #131493

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and mEos3.2 donor (Camk2a, Camk2a Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaV1.3e[8a,11,31b,Δ32,Δ42a]

   Plasmid

   #49332

   Purpose

   Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

   Promoter

   CMV

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TrkC-GFP

   Plasmid

   #24094

   Depositor

   Moses Chao

   Insert

   TrkC (Ntrk3 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

9. miniCGBE1-VRQR (pBM1173)

   Plasmid

   #140255

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_VRQR-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

    Plasmid

    #62044

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LAP2beta (Tmpo Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDC158

    Plasmid

    #115793

    Purpose

    Expresses Rat SNAP25

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    SNAP25 (Snap25 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    c84s, c85s, c90s, c92s

    Promoter

    T7

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Stx1a-12aa-mCer

    Plasmid

    #53236

    Purpose

    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    Syntaxin 1A (Stx1a Rat)
    

    Use

    Tags

    12aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST1374-scFv-APOBEC-UGI-GB1

    Plasmid

    #113029

    Purpose

    Expresses scFv-rAPOBEC1-UGI in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    scFv-rAPOBEC1-UGI-GB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Erk2

    Plasmid

    #29582

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    HIS6 and TEV

    Expression

    Bacterial

    Mutation

    Starting Met removed from Erk2

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF Synaptophysin-GCamp6s

    Plasmid

    #188981

    Purpose

    Presynaptic fluorescent Ca2+ indicator

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Rat, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

    Plasmid

    #131508

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CD200RCD4d3+4-bio-linker-His

    Plasmid

    #36153

    Purpose

    A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    CD200R (Cd200r1 Rat)
    

    Use

    Tags

    His tag, biotinylation peptide, and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. VV229: GCaMP6f in fck

    Plasmid

    #58514

    Purpose

    Expresses GCaMP6f under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Tags

    C-terminal FCYENEV (ER export motif); this was no…

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA type III TGF beta receptor (R3-OFF)

    Plasmid

    #10913

    Depositor

    Bob Weinberg

    Article

    Wang et al Cell. 1991 Nov 15. 67(4):797-805.

    Insert

    R3-OFF (Tgfbr3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

23. sfGFP-Lysosomes-20

    Plasmid

    #56487

    Purpose

    Localization: Lysosome Membrane, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pRK7-HA-S6K1-F5A

    Plasmid

    #8986

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pLVX-CMV-Galpha-i3 G183S ONE-GO

    Plasmid

    #204338

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-i3 G183S ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G183S mutation in Galpha-i3 (renders it RGS insen…

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pCI-syn-fRGECO2

    Plasmid

    #125244

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W44Y, D432A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-C2gamma

    Plasmid

    #21215

    Depositor

    Tobias Meyer

    Article

    Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

    Insert

    GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The C2-domain from PKCgamma (aa170-260) plus flan…

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KIF5C(1-560)-2xmCherry-EF(C)

    Plasmid

    #61664

    Purpose

    Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cav2.1 HA pCAGGS

    Plasmid

    #206076

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3XFLAG-CMV 7.1_syn6

    Plasmid

    #50012

    Purpose

    Encodes syntaxin 6 with a N-terminal 3X FLAG tag

    Depositor

    David Hackstadt

    Article

    Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

    Insert

    syntaxin 6 (Stx6 Rat)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge