Skip to main content
Addgene
Showing: 801 - 840 of 847 results

  5. pDGE792

    Plasmid
    #167300
    Purpose
    Vector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167300-plasmid-map-sequence-id-333327

    Enlarge

  7. pAM5188

    Plasmid
    #120087
    Purpose
    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120087-plasmid-map-sequence-id-234428

    Enlarge

  11. AA390

    Plasmid
    #215957
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control reporter
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgEGFP_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215957-plasmid-map-sequence-id-431547

    Enlarge

  17. pDiCre-mCherry

    Plasmid
    #164572
    Purpose
    Integration of a DiCre cassette in PbGFP parasite genome
    Depositor
    Olivier Silvie
    Article
    Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d
    Inserts
    FKBP12-CRE59
    FRB-CRE60
    mCherry
    Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164572-plasmid-map-sequence-id-325185

    Enlarge

  19. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

    Plasmid
    #179402
    Purpose
    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)
    Depositor
    Tuncay Baubec
    Article
    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038
    Insert
    CBX7_Chromo - TAF3_Phd(DW890/891AA)
    Use
    Tags
    AviTag and EGFP
    Expression
    Mammalian
    Mutation
    Phd domain DW890/891AA
    Promoter
    CAGGS
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    179402-plasmid-map-sequence-id-370528

    Enlarge

  20. prCOrev

    Plasmid
    #153961
    Purpose
    Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
    Use
    Retroviral; A reporter plasmid detecting dna reco…
    Tags
    NLS-ZF
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    LTR
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153961-plasmid-map-sequence-id-306376

    Enlarge

  21. prCO

    Plasmid
    #153960
    Purpose
    Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
    Use
    Retroviral; A reporter plasmid detecting dna reco…
    Tags
    NLS-ZF
    Expression
    Mammalian
    Mutation
    No mutation
    Promoter
    LTR
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153960-plasmid-map-sequence-id-306370

    Enlarge

  22. lentiGuide-chr20-49345720-gRNA1

    Plasmid
    #232623
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232623-plasmid-map-sequence-id-465706

    Enlarge

  23. lentiGuide-chr20-49345720-gRNA2

    Plasmid
    #232624
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232624-plasmid-map-sequence-id-465707

    Enlarge

  24. lentiGuide-chr20-49345720-gRNA3

    Plasmid
    #232625
    Purpose
    Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.
    Depositor
    Claes Wadelius
    Article
    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1
    Insert
    S. pyogenes sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    April 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232625-plasmid-map-sequence-id-465708

    Enlarge

  26. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

    Enlarge

  31. Ma-pREP

    Plasmid
    #197571
    Purpose
    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    111TAG and 8TAG/112TAG
    Promoter
    AraC, T7, cat promoter (constitutive), and lpp (c…
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197571-plasmid-map-sequence-id-389424

    Enlarge

  32. pCAG-VHC

    Plasmid
    #112618
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112618-plasmid-map-sequence-id-222032

    Enlarge

  33. pCAG-tdTHC

    Plasmid
    #112621
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112621-plasmid-map-sequence-id-222036

    Enlarge

  34. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

    Enlarge

  35. ptdTHC

    Plasmid
    #112617
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112617-plasmid-map-sequence-id-224553

    Enlarge

  36. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

    Enlarge

  37. pCAG-EHC

    Plasmid
    #112619
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112619-plasmid-map-sequence-id-226490

    Enlarge

  38. pEHC

    Plasmid
    #112615
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Emerald
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112615-plasmid-map-sequence-id-226350

    Enlarge

  39. pmTHC

    Plasmid
    #112616
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112616-plasmid-map-sequence-id-224548

    Enlarge

  40. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

    Enlarge

Showing: 801 - 840 of 847 results