We narrowed to 1,334 results for: piggyBac

1. pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

   Plasmid

   #233067

   Purpose

   Piggybac CRISPRi construct

   Depositor

   Markus Landthaler

   Article

   Vučićević et al bioRxiv 2024.09.12.612198

   Insert

   KRAB-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YX022 (PB)EF1a-LIDARsensor (hygR)

   Plasmid

   #233431

   Purpose

   EF1a-driven LIDAR reporter RNA

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1a

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB-cT3G-cERP2-BRCA1

   Plasmid

   #222530

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of BRCA1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-cT3G-cERP2-HMGB1

   Plasmid

   #222534

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HMGB1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

   Plasmid

   #195567

   Purpose

   PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHM148

   Plasmid

   #225532

   Purpose

   Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Inserts

   rtTA
   Pcdh19 (Pcdh19 Mouse)
   mCherry
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-cT3G-cERP2-SOD1

   Plasmid

   #222568

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB-cT3G-cERP2-RB1

   Plasmid

   #222550

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of RB1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   RB1 (RB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB-cT3G-cERP2-KDM5B

   Plasmid

   #222555

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of KDM5B

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   KDM5B (KDM5B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Xlone Puro SNAP-eGFP-BASU

    Plasmid

    #218452

    Purpose

    expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integration

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    SNAP-eGFP-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI

    Plasmid

    #202596

    Purpose

    plasmid for expressing the DNMT3bCI (catalytic inactive)

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Synthetic, Mouse)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    P656V, C657D

    Promoter

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

    Plasmid

    #167899

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

    Plasmid

    #167897

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

14. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

    Plasmid

    #167903

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

15. BicC.Ca9.R

    Plasmid

    #168295

    Purpose

    Expresses Cas9 in Drosophila late germline cells

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    BicC.Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto

    Plasmid

    #167896

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

    Plasmid

    #162815

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_M
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_M(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

    Plasmid

    #162816

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_9b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_9b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

    Plasmid

    #162821

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b_alt
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b_alt(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BII-C3H

    Plasmid

    #133386

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BII-Sh-PnGW-CMV

    Plasmid

    #133366

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BII-ChPtW-eSpCas9

    Plasmid

    #133381

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BII-Sh-PnGW-CMV-Luc

    Plasmid

    #133367

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BII-Sh-PIR702W-Luc

    Plasmid

    #133362

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IRFP-702
    

    Use

    Tags

    HA-tagged IRFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BII-Sh-PIR702W

    Plasmid

    #133361

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IRFP-702
    

    Use

    Tags

    HA-tagged IRFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BII-Sh-PnGW-Luc

    Plasmid

    #133360

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Use

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

    Plasmid

    #124169

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

    Plasmid

    #124175

    Purpose

    piggyBac-based Dox-inducible expression (very weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

    Plasmid

    #124174

    Purpose

    piggyBac-based Dox-inducible expression (weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. KA1123_pPB-TREtight-cHA-IRESH2BmKO2

    Plasmid

    #124172

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB-TO-ASCL1-DLX2

    Plasmid

    #182307

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pTRE3G-bidirectional

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205555

    Purpose

    Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge