We narrowed to 1,177 results for: POLI

1. pCellFree_G03 BMPR1A

   Plasmid

   #67105

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BMPR1A (BMPR1A Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCellFree_G03 EHD2

   Plasmid

   #67107

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EHD2 (EHD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 GTF2H1

   Plasmid

   #67108

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2H1 (GTF2H1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEMHE-mH33B-mClover3

   Plasmid

   #171496

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   H3.3B (H3f3b Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRMC2-sec:msfGFP

   Plasmid

   #194914

   Purpose

   Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEMHE-mClover3-KASH5-DN

   Plasmid

   #171492

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEMHE-GST-KASH5-DN

    Plasmid

    #171493

    Purpose

    T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    GST

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid

    #89886

    Purpose

    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-NRasQ61K
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid

    #89887

    Purpose

    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-HRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PVRL2-bio-His

    Plasmid

    #51824

    Purpose

    Expresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PVRL2 (NECTIN2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD21-hgAPOAI

    Plasmid

    #124962

    Purpose

    Express ApoAI in the liver

    Depositor

    Kazuhiro Oka

    Article

    Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

    Insert

    human APOAI gene (LPA Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    human APOAI

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-KLF3

    Plasmid

    #49100

    Purpose

    Expresses KLF3 in mammalian cells

    Depositor

    Merlin Crossley

    Article

    Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

    Insert

    Krüppel-Like Factor 3(KLF3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

18. Str-KDEL_SBP-EGFP-CCR5

    Plasmid

    #222309

    Purpose

    Synchronize the trafficking of CCR5 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Str-KDEL_SBP-mCherry-CCR1

    Plasmid

    #222311

    Purpose

    Synchronize the trafficking of CCR1 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCRII apoBb.1

    Plasmid

    #121898

    Purpose

    Plasmid for making zebrafish apoBb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBb.1 (apobb.1 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PCRII apoA-IVb.1

    Plasmid

    #121903

    Purpose

    Plasmid for making zebrafish apoA-IVb.1 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.1 (apoa4b.1 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PCRII apoA-IVb.2

    Plasmid

    #121904

    Purpose

    Plasmid for making zebrafish apoA-IVb.2 in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoa4b.2 (apoa4b.2 Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEMHE-mScarlet-hCenpC

    Plasmid

    #171490

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    CenpC (CENPC Human)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid

    #107581

    Purpose

    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PCRII apoEb

    Plasmid

    #121901

    Purpose

    Plasmid for making zebrafish apoEb in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    Apoeb (apoeb Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-DN-RFX

    Plasmid

    #52296

    Purpose

    Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Dominant negative RFX
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCRII apoEa

    Plasmid

    #121900

    Purpose

    Plasmid for making zebrafish apoEa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoEa (apoea Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Str-KDEL_SBP-EGFP-CCR5-Cys3A

    Plasmid

    #222313

    Purpose

    Synchronize the trafficking of CCR5-Cys3A from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C321A, C323A, C324A

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEMHE-mClover3-bElys

    Plasmid

    #171494

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Elys mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Elys (AHCTF1 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PCRII apoBa

    Plasmid

    #121897

    Purpose

    Plasmid for making zebrafish apoBa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBa (apoba Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EGFP-BRCA1

    Plasmid

    #115491

    Purpose

    EGFP-BRCA1 expression vector. Parton clone BXY

    Depositor

    Rob Parton

    Article

    McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1009R (See depositor comments)

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEMHE-mScarlet-MAP4-MTBD

    Plasmid

    #171488

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-hGPR56-IRES-GFP

    Plasmid

    #52297

    Purpose

    Expresses human GPR56 and GFP in mammalian cells.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Human GPR56 coding sequence (ADGRG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEMHE-miRFP-MAP4-MTBD

    Plasmid

    #171487

    Purpose

    T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    miRFP

    Expression

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1+/-FLI1-C‐(K)‐DYK

    Plasmid

    #129497

    Purpose

    Mammalian expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid

    #89890

    Purpose

    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    HA-BirA-2A-citrine
    

    Use

    Unspecified

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Cherry-SRBC

    Plasmid

    #69492

    Purpose

    Expression of fluorescently tagged SRBC in mammalian cells

    Depositor

    Rob Parton

    Article

    Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

    Insert

    SRBC (Cavin3 Mouse)
    

    Use

    Tags

    Cherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

40. mCherry-PARP1

    Plasmid

    #215136

    Purpose

    Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PB-PARP1-del.p119K120S–eGFP

    Plasmid

    #211554

    Purpose

    A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 119K 120S

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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43. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. pTRIPdU3-MND-FLAG-FLI1-IRES-GFP

    Plasmid

    #129496

    Purpose

    Lentiviral expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    MND

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pDECKO_GFP

    Plasmid

    #141210

    Purpose

    empty GFP vector for cloning sgRNA or pgRNAs

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSPL3-hRPH3A_c.1853Gmut

    Plasmid

    #190479

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.1853A>G

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107576

    Purpose

    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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