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Showing: 1001 - 1050 of 4900 results

  1. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210734
    Purpose
    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Frodo sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    210734-plasmid-map-sequence-id-464595

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  2. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210735
    Purpose
    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Gollum sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210735-plasmid-map-sequence-id-420080

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  3. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210736
    Purpose
    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Merry sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210736-plasmid-map-sequence-id-420081

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  5. pUK21

    Plasmid
    #49788
    Purpose
    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)
    Depositor
    Joachim Messing
    Article
    Vieira et al Gene. 1991 Apr;100:189-94.
    Type
    Empty backbone
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lacZ
    Available since
    July 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49788-plasmid-map-sequence-id-160334

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  9. pMSCVsin-PGK-GFP

    Plasmid
    #35615
    Depositor
    Patrick Salmon
    Insert
    GFP
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    35615-plasmid-map-sequence-id-321487

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  10. pMSCVsin-PGK-PURO

    Plasmid
    #35618
    Depositor
    Patrick Salmon
    Insert
    Puro
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    35618-plasmid-map-sequence-id-43780

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  11. EF1a-EGFP (no signal control)

    Plasmid
    #170167
    Purpose
    Mammalian Expression of no signal control
    Depositor
    Christopher Moxham
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    170167-plasmid-map-sequence-id-338528

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  12. w+attB

    Plasmid
    #30326
    Depositor
    Jeff Sekelsky
    Insert
    phiC31 attB, hsp70::w
    Use
    Drosophila transgenesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    30326-plasmid-map-sequence-id-398304

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  13. EF1a-EGFP-SKL

    Plasmid
    #170166
    Purpose
    Mammalian Expression of Ef1a-EGFP-SKL
    Depositor
    Christopher Moxham
    Insert
    EGFP-SKL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SKL
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170166-plasmid-map-sequence-id-337495

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  14. EF1a-EGFP-RSKL

    Plasmid
    #170165
    Purpose
    Mammalian Expression of Ef1a-EGFP-RSKL
    Depositor
    Christopher Moxham
    Insert
    EGFP-RSKL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    RSKL
    Promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    170165-plasmid-map-sequence-id-337503

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  15. EF1a-EGFP-KANL

    Plasmid
    #170164
    Purpose
    Mammalian Expression of Ef1a-EGFP-KANL
    Depositor
    Christopher Moxham
    Insert
    EGFP-KANL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    KANL
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170164-plasmid-map-sequence-id-337496

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  16. EF1a-ABCD3-RFP-Puro

    Plasmid
    #170168
    Purpose
    Mammalian Expression of Ef1a-ABCD3-RFP-Puro
    Depositor
    Christopher Moxham
    Insert
    ABCD3-RFP-Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    170168-plasmid-map-sequence-id-334410

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  17. DONOR plasmid

    Plasmid
    #236552
    Purpose
    Contains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236552-plasmid-map-sequence-id-469275

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  18. pTU2-b_UK_amilCP

    Plasmid
    #204064
    Purpose
    Type TU2-b backbone with kanamycin resistance and pUC ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    204064-plasmid-map-sequence-id-451677

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  19. pTU1-A_UK_amilCP

    Plasmid
    #204043
    Purpose
    Type TU1-A backbone with kanamycin resistance and pUC ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204043-plasmid-map-sequence-id-418161

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  20. pSAH031

    Plasmid
    #90330
    Purpose
    Promoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.
    Depositor
    David Savage
    Insert
    iLOV
    Use
    Tags
    None
    Expression
    Mutation
    Promoter
    None
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    90330-plasmid-map-sequence-id-189687

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  21. pTU2-b_UrK_amilCP

    Plasmid
    #204065
    Purpose
    Type TU2-b backbone with kanamycin resistance and pUC-bom-rop ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204065-plasmid-map-sequence-id-418194

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  22. pTU1-A_UrK_amilCP

    Plasmid
    #204044
    Purpose
    Type TU1-A backbone with kanamycin resistance and pUC-bom-rop ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204044-plasmid-map-sequence-id-418162

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  24. CRISPR plasmid

    Plasmid
    #236553
    Purpose
    Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Cas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236553-plasmid-map-sequence-id-469881

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  25. StarShip01

    Plasmid
    #164505
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164505-plasmid-map-sequence-id-324722

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  26. StarShip02

    Plasmid
    #164506
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164506-plasmid-map-sequence-id-324723

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  27. StarShip03

    Plasmid
    #164507
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164507-plasmid-map-sequence-id-324724

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  29. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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  30. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Use
    Tags
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

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  36. Cas9-T2A-H2B mCitirine

    Plasmid
    #210471
    Purpose
    Coexpression of Cas9 and H2B mCitrine
    Depositor
    Amro Hamdoun
    Insert
    Cas9-T2A-H2B mCitirine
    Use
    CRISPR and Synthetic Biology
    Tags
    mCitrine on C terminal of H2B
    Expression
    Mammalian
    Mutation
    Cas9 contains 3xFLAG tag and nuclear localization…
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210471-plasmid-map-sequence-id-416284

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  46. KG#865

    Plasmid
    #110912
    Purpose
    Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
    Depositor
    Kenneth Miller
    Insert
    Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110912-plasmid-map-sequence-id-212549

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  47. KG#883

    Plasmid
    #110913
    Purpose
    Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein
    Depositor
    Kenneth Miller
    Insert
    5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110913-plasmid-map-sequence-id-212540

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Showing: 1001 - 1050 of 4900 results