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We narrowed to 3,102 results for: sv40

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Showing: 1001 - 1050 of 3102 results

  6. Ir7a-T2A-QF2 HDR plasmid

    Plasmid
    #140943
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140943-plasmid-map-sequence-id-313597

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  7. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid
    #101191
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5(K918A/V922A/R925A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101191-plasmid-map-sequence-id-194254

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  8. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid
    #101187
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101187-plasmid-map-sequence-id-194250

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  9. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid
    #101189
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101189-plasmid-map-sequence-id-194252

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  10. pGL3 Control Vector

    Plasmid
    #212937
    Purpose
    Control vector with constitutive firefly luciferase expression
    Depositor
    Debrya Groskreutz
    Insert
    luc+
    Use
    Luciferase
    Mutation
    WT
    Promoter
    SV40
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212937-plasmid-map-sequence-id-419191

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  11. pGL3 Promoter Vector

    Plasmid
    #212939
    Purpose
    Control vector with constitutive firefly luciferase expression
    Depositor
    Debrya Groskreutz
    Insert
    luc+
    Use
    Luciferase
    Mutation
    WT
    Promoter
    SV40
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212939-plasmid-map-sequence-id-419192

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  12. NLS-tdTomato

    Plasmid
    #163443
    Purpose
    Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone
    Depositor
    Sanford Simon
    Insert
    tdTomato
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163443-plasmid-map-sequence-id-321654

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  13. pBabepuro-TGFA

    Plasmid
    #189610
    Purpose
    Retroviral expression vector for TGFA
    Depositor
    Thierry Tchenio
    Insert
    TGFA (TGFA Human)
    Use
    Retroviral
    Promoter
    SV40 early promoter
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189610-plasmid-map-sequence-id-381090

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  14. H_NLS 41kDa (BFP)

    Plasmid
    #201391
    Purpose
    Nucleocytoplasmic transport probe
    Depositor
    Pere Roca-Cusachs
    Insert
    (NLS)SV40-BFP-2PrA
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201391-plasmid-map-sequence-id-399879

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  15. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Gateway Cloning and Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41390-plasmid-map-sequence-id-352470

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  16. pDLucBiDi

    Plasmid
    #219562
    Purpose
    Dual luciferase plasmid with Renilla luciferase constitutively expressed and firefly luciferase downstream of MCS without a start codon
    Depositor
    Pasha Baranov
    Type
    Empty backbone
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40 and T7
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    219562-plasmid-map-sequence-id-439245

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  17. pGGD_RT_li_Tq2CFP_2NLS

    Plasmid
    #136972
    Purpose
    Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Expression
    Bacterial
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136972-plasmid-map-sequence-id-269108

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  22. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  24. pCAG_iPE-N

    Plasmid
    #214734
    Purpose
    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-N_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214734-plasmid-map-sequence-id-453897

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  26. pCAG_iPE-C

    Plasmid
    #214735
    Purpose
    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-C_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214735-plasmid-map-sequence-id-425000

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  28. IR25a-T2A-QF-AD

    Plasmid
    #162526
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162526-plasmid-map-sequence-id-354666

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  39. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

    Plasmid
    #101177
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101177-plasmid-map-sequence-id-194000

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  41. ORCO-T2A-QF2

    Plasmid
    #162524
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162524-plasmid-map-sequence-id-354668

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  44. pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR

    Plasmid
    #202830
    Purpose
    pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    CHAF1B intronic SVA del-VNTR sequence
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202830-plasmid-map-sequence-id-399708

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  45. IR76b-T2A-QF2

    Plasmid
    #162523
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162523-plasmid-map-sequence-id-354669

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  46. GR3-T2A-QF2

    Plasmid
    #162521
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162521-plasmid-map-sequence-id-372762

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  47. IR8a-T2A-QF2

    Plasmid
    #162520
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162520-plasmid-map-sequence-id-372758

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  49. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid
    #141100
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    Use
    Donor template
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141100-plasmid-map-sequence-id-292288

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  50. IR25a-T2A-QF2

    Plasmid
    #162522
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162522-plasmid-map-sequence-id-354640

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Showing: 1001 - 1050 of 3102 results