59,540 results

1. pLV-ATF5-PROX1-HNF1A

   Plasmid

   #149725

   Purpose

   expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

   Depositor

   Baisong Lu

   Article

   Lu et al Biomaterials. 2021 Feb;269:120668. doi:

   Insert

   ATF5-PROX1-HNF1A (ATF5 Human)
   

   Use

   Lentiviral

   Tags

   P2A, T2A

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hATG7wt

   Plasmid

   #87867

   Purpose

   Express a wild type human Atg7/Apg7 in mammalian cells

   Depositor

   Eiki Kominami, Isei Tanida

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   ATG7 (ATG7 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EML4-ALK variant 3a I1171N/D1203N

   Plasmid

   #183833

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   I1171N/D1203N

   Promoter

   CMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458M-53BP1-DN1S

   Plasmid

   #131045

   Purpose

   Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

   Depositor

   Punam Malik

   Article

   Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

   Insert

   SpCas9-53BP1-DN1S (TP53BP1 Human)
   

   Use

   CRISPR

   Tags

   GFP and SpCas9

   Expression

   Mammalian

   Mutation

   Only expressing residues 1231-1644 of complete 53…

   Promoter

   CBH

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAuroraA-GFP-AURKA

   Plasmid

   #99877

   Purpose

   Expression of GFP-AURKA in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   AURKA minimal

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MELOPS

   Plasmid

   #80151

   Purpose

   Genetically encoded melanosome-localized pH sensor.

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   OCA2 (OCA2 Human)
   

   Tags

   mNectarine in first luminal loop

   Expression

   Mammalian

   Mutation

   nonpathologic polymorphism D257A, pore mutation V…

   Promoter

   CMV

   Available Since

   Sept. 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pinducer 20 DN-KASH

   Plasmid

   #125554

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Mutation

   Truncated form: only the KASH domain of nesprin-…

   Promoter

   tetracycline responsive element

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. W118-1_hTGM2-Flag

   Plasmid

   #180407

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVTNT-T7-KPNA4

   Plasmid

   #26680

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 4 (KPNA4 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human USP7-3X FLAG

    Plasmid

    #225334

    Purpose

    Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-sfCherry11x4-ß-actin

    Plasmid

    #70220

    Purpose

    Expresses sfCherry11x4-ß-actin in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    sfCherry11x4-actin (ACTB Human)
    

    Tags

    sfCherry11x4

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. opto-E-cad GFP

    Plasmid

    #203327

    Purpose

    Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

    Depositor

    Seraphine Wegner

    Article

    Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

    Insert

    Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Addition of Aslov2 domain at T133 of human E-cadh…

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNL-CEF-BAFFR-CFP

    Plasmid

    #187002

    Purpose

    Expression of human BAFFR-CFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    BAFFR (TNFRSF13C Human)
    

    Use

    Lentiviral

    Tags

    CFP

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104907

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0794

    Plasmid

    #142239

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF7 (ATF7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC7A9

    Plasmid

    #132245

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A9 (SLC7A9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. ADORA1-Tango

    Plasmid

    #66209

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADORA1 (ADORA1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. EF1a_MYC_P2A_Hygro_Barcode

    Plasmid

    #120461

    Purpose

    Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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