We narrowed to 26,822 results for: STI

1. pXPR_053

   Plasmid

   #113591

   Purpose

   Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

   Insert

   sgRNA cloning site
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SBP-GFP-Tac

   Plasmid

   #162505

   Purpose

   Expresses GFP tagged Tac in a RUSH vector in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   Tac (IL2RA Human)
   

   Tags

   GFP and SBP

   Expression

   Mammalian

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFlox_N-Nipbl_Neo_FKBPF36V_mScarlet

   Plasmid

   #163883

   Purpose

   Acute Depletion NIPBL-mScarlet

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Nipbl (Nipbl Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

   Plasmid

   #203844

   Purpose

   Flp-dependent Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-GFP-ATL3

   Plasmid

   #109024

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   EIF-1a promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

   Plasmid

   #170578

   Purpose

   Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDKL5 (CDKL5 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   E854G- please see depositor comments

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1/GgVcl 1-1066 T12 mutant

   Plasmid

   #46266

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

   Insert

   Vcl T12 mutant (VCL Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T12 mutant (D974;K975;R976; R978A) autoinhibition…

   Promoter

   CMV

   Available Since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn FLEx-OFF hM4Di-mCherry

   Plasmid

   #161579

   Purpose

   To express hM4Di in cells not expressing Cre.

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM4Di-mCherry
   

   Use

   AAV

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRS-AraC-GFP

   Plasmid

   #220199

   Purpose

   mini-Tn7 GFP expression vector - AraC/pBAD promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   monomeric superfolder green fluorescent protein
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   AraC/pBAD

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRS-XylS-GFP

    Plasmid

    #220200

    Purpose

    mini-Tn7 GFP expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    XylS/pM

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. NET33 pEGFP-N2 (597)

    Plasmid

    #61989

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1.ARRDC1

    Plasmid

    #38320

    Depositor

    Quan Lu

    Article

    Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    ARRDC1 (ARRDC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. LC001: pMVP/PB/Hygro-DEST

    Plasmid

    #121876

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cdc2-DN-HA

    Plasmid

    #1889

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdc2 (CDK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D146N, dominant negative

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcCas12nHS_ABE_D3_empty

    Plasmid

    #203809

    Purpose

    Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    ABE_D3
    

    Use

    CRISPR

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

    Plasmid

    #203838

    Purpose

    Flp-dependent Cre and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mScarlet-KASH-2A

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Tag4A-MITF-M (wt)

    Plasmid

    #31151

    Depositor

    Yardena Samuels

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    MITF-M (MITF Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-TetO-PM

    Plasmid

    #197387

    Purpose

    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    PDX1-T2A-MAFA
    

    Use

    Lentiviral; Teto

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

    Plasmid

    #32520

    Depositor

    Mustafa Sahin

    Article

    Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

    Insert

    Constitutively active Rheb (S16H) (Rheb Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S16H to make constitutively active

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LB601: pMVP/PB/eGFP-DEST

    Plasmid

    #121870

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV-mEGFP(A206K)-paCaMKII-ShadowG

    Plasmid

    #167475

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII-ShadowG
    

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GreenT-EC - Display - CyRFP1

    Plasmid

    #193943

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-flag-mKdm4d-polyA

    Plasmid

    #61553

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (Kdm4d Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #133786

    Purpose

    Cre-dependent tdTomato fluorescent reporter for cell labeling

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    pAAV-EF1a-DIO-tdTomato-WPRE
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ypet-PBD

    Plasmid

    #105290

    Purpose

    To measure Rac or Cdc42 activity by binding and FRET analysis

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    aa encoding 65-150

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET29b-pduCDE

    Plasmid

    #110138

    Purpose

    Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

    Insert

    pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5 FRT/TO Plk4 ∆24-mCherry

    Plasmid

    #80271

    Purpose

    mammalian expression plasmid for Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFastBac1 Flag BRG1

    Plasmid

    #1957

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BRG1 (SMARCA4 Human)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKLV-PGKpuro2ABFP

    Plasmid

    #122372

    Purpose

    Expresses puro-2A-BFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    Puro-2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSF-G1(7AW)RS

    Plasmid

    #207516

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV hSyn FLEx-OFF hM3Dq-mCherry

    Plasmid

    #161578

    Purpose

    To express hM3Dq in cells not expressing Cre.

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YFP-Cdc42(V12)

    Plasmid

    #11399

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    cell division cycle 42 (CDC42 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glycine 12 changed to Valine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR221-hSOX10

    Plasmid

    #24749

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Mutation

    wildtype sequence

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

40. REEP5-mEmerald

    Plasmid

    #186964

    Purpose

    Express REEP5/DP1 with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Reep5 (Reep5 Mouse)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge