We narrowed to 17,163 results for: Emb;
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Plasmid#70179PurposeMammalian expression of hsOPA1 isoform 7DepositorAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only
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pSIN-TRE-EB3N-VVDfast-mCl3-iLID
Plasmid#190169PurposeDoxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseLentiviralTags-VVDfast-mClover3-iLIDMutation1-189 aa from EB3PromoterTREAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 p37
Plasmid#169015PurposeExpresses GST-p37 (human, codon optimized for expression in bacteria)DepositorInsertp37 (UBXN2B Human)
TagsGSTExpressionBacterialMutationsequence was codon optimized for expression in E.…PromotertacAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2w/b
Plasmid#111570PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-E-cadherin promoter
Plasmid#61798PurposeLuciferase reporter containing the mouse E-cadherin promoterDepositorInsert-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
UseLuciferaseExpressionMammalianAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2
Plasmid#167295PurposePlasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherryDepositorInsertBAK (BAK1 Human)
ExpressionMammalianAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
Plasmid#176395PurposeExpresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.DepositorInsertGAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
ExpressionYeastPromoterGAL1Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Actin-mRFP-PAGFP
Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorAvailable SinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Puro-H2BC11
Plasmid#207761PurposeDonor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralExpressionMammalianPromoterHuman EF1-alphaAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-mmCdh5pro (JDW 1239)
Plasmid#224478PurposeGateway compatible 5' entry clone containing the Murine Cdh5 promoterDepositorInsertCdh5 promoter
ExpressionMammalianAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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eiCBE
Plasmid#227148PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoterDepositorInsertbpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
MutationD96R/E84R/V159R/H339APromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Il12b 400bp pro-Luc
Plasmid#20020DepositorAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
AA183
Plasmid#216025PurposeFragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiencyDepositorHas ServiceCloning Grade DNAInsertnCas9 (H840A)_v2.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
ACE2(-1119)-luc
Plasmid#31110PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseExpressionMammalianMutationpromoter region -1119 to +103Available SinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
ExpressionMammalianAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER
Plasmid#113898PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligaseDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6PromoterCMV-MIE-TetO2Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Jag1-HA pIRES
Plasmid#17336DepositorAvailable SinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-MAPRE1
Plasmid#207793PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.DepositorInsertsgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-CEP135
Plasmid#227286PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.DepositorInsertsgRNA Targeting N-terminus of CEP135 (CEP135 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-hPAC-FLAG
Plasmid#129494PurposeExpresses human TMEM206 tagged at the C-terminus with FLAGDepositorInserthuman PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
TagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37deltaSEP
Plasmid#113501PurposeBacterial expression of GST-tagged p37 lacking the SEP domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 140-206Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP
Plasmid#138334PurposeExpresses APOB-Linker-eGFPDepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-ERT2-iCre-ERT2
Plasmid#178060PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.DepositorInsertERT2-iCre-ERT2
UseAAVExpressionMammalianPromoterHuman ubiquitin C (UBC) promoterAvailable SinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_hph
Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
ExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable SinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh
Plasmid#31847PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianPromoterU6 for shRNA; Ef1alpha for mCherryAvailable SinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Sox2-IRES-Neo
Plasmid#13462DepositorAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST
Plasmid#109048PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vectorDepositorHas ServiceAAV9InserteGtACR1-ST
UseAAVPromoterCAGAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Blast-H2BC11
Plasmid#207757PurposeDonor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p5E-hs_ICAM_short (JDW 1046)
Plasmid#224480PurposeGateway compatible 5' entry clone containing the Human minimal ICAM2 promoterDepositorInsertmin-ICAM2 promoter
ExpressionMammalianAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rat Alpha2 delta-1 HA pMT2
Plasmid#58729Purposeexpression of HA tagged rat Alpha2 delta-1 in mamalian cellsDepositorAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvHA
Plasmid#234991PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinsDepositorInsertNA-3C-myc-scfvHA
ExpressionMammalianAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-Sintbad
Plasmid#234668Purposeexpresses SINTBAD (TBKBP1) in mammalian cellsDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsEDC4
Plasmid#66597PurposeExpresses lambdaN-HA-tagged EDC4 in mammalian cellsDepositorAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKH151_PB_flag-LC3B_wt_IRES-BFP
Plasmid#214976PurposeFlag tagged LC3BDepositorInsertFLAG-LC3B (MAP1LC3B Human)
ExpressionMammalianAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAG43
Plasmid#226736PurposeExpresses a reporter for an alpha-helical OMM proteinDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-TGAT
Plasmid#84334PurposeExpresses TGAT tagged with mCherryDepositorAvailable SinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-INPP4B
Plasmid#116864PurposeRecruitable PI(3,4)P2 4-phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-mSin1(1.1)-HA
Plasmid#73388PurposeExpresses mSin-HADepositorInsertmSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
TagsHA tagExpressionMammalianPromoterCMVAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-PXN
Plasmid#227317PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorInsertPXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP2A-mNeonGreen
Plasmid#228525PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.DepositorInsertLAMP2 (Lamp2 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianPromoterUbcAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11
Plasmid#227332PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CBh-dCas9-hEzh2-IRES-Hyg
Plasmid#122375PurposeExpresses dCas9-hEzh2-IRES-Hyg in mammalian cellsDepositorInsertCBh-dCas9-hEzh2-IRES-Hyg-pA
UsePiggybacExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-actin prom-dsRed-scramble shRNA
Plasmid#67879PurposeAAV vector to express dsRed sh RNA (scramble)DepositorInsertactin prom-dsRed-scramble shRNA
UseAAV and RNAiExpressionMammalianPromoterchicken beta actinAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKH152_PB_flag-LC3B_LDSmut_IRES-BFP
Plasmid#214977PurposeFlag tagged LDS mutant LC3BDepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only