164,577 results

1. pMulti_S_ccdB

   Plasmid

   #223853

   Purpose

   GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Type

   Empty backbone

   Use

   Golden-gate acceptor

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

   Plasmid

   #218973

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant SRSF1 binding motif (AGTCTCCT)

   Promoter

   RSV LTR

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG426-GAL-Kar2-Beta-Amyloid

   Plasmid

   #181707

   Purpose

   Galactose inducible expression of Kar2-Beta-Amyloid

   Depositor

   Alejandro Chavez

   Insert

   Kar2-Beta-Amyloid
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-ORP8-H514A-H515A

   Plasmid

   #208360

   Purpose

   Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   ORP8 (OSBPL8 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H514A-H515A

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_CRFmut

   Plasmid

   #208656

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-DCX-TurboID-V5

   Plasmid

   #190740

   Purpose

   Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   doublecortin (DCX Human)
   

   Use

   Tags

   TurboID and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgKeap1#1/Cre

   Plasmid

   #173639

   Purpose

   Expresses a Keap1-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Keap1 (Keap1 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Promoter

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-TagRFP657

   Plasmid

   #176563

   Purpose

   Expression of TagRFP657 for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZBTB4-donor

    Plasmid

    #176374

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZBTB4 (ZBTB4 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDM326_DPAKa(GBD)-YFP

    Plasmid

    #128701

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Expression

    Mutation

    none

    Promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111923

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiPGK Blast DEST JNKKTRmRuby2

    Plasmid

    #59154

    Purpose

    Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    mCherry*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Use

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi G418R mbIL21

    Plasmid

    #194329

    Purpose

    Consitiutive mammalian expression of membrane bound human IL-21

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    mbIL-21 (IL21 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK435

    Plasmid

    #236573

    Purpose

    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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