We narrowed to 31,552 results for: Eng;

1. pHR_TRE3GS-->sGFP-P2A-mCherry

   Plasmid

   #162232

   Purpose

   Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   EGFP and mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV_psychLight2

   Plasmid

   #163910

   Purpose

   Expresses psychLight2 in mammalian cells

   Depositor

   Lin Tian

   Article

   Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

   Insert

   psychLight2
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

   Plasmid

   #208378

   Purpose

   Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human CMV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

   Plasmid

   #208379

   Purpose

   Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human CMV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDestTol2-QUAS:GCaMP7a

   Plasmid

   #184817

   Purpose

   Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QUAS:GCaMP7a-pA
   

   Use

   Zebrafish tol2 transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRES2-eGFP-Ofd1-Myc

   Plasmid

   #24560

   Depositor

   Jeremy Reiter

   Article

   Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXN21-Dest

   Plasmid

   #105086

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Use

   Tags

   NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIP_RNA2FLAG

   Plasmid

   #61261

   Purpose

   Flag tagged Orsay virus RNA2 genome segment expression plasmid

   Depositor

   David Wang

   Article

   Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

   Insert

   Orsay RNA2
   

   Use

   Tags

   Flag fused

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJW1311

   Plasmid

   #61254

   Purpose

   Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E) targeting Y61A9L9.1
   

   Use

   CRISPR; Cloning template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRESneo-FLAG/HA Ago4

    Plasmid

    #10824

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 4 (AGO4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCM4.4

    Plasmid

    #217648

    Purpose

    an ermE*p-cas9-driven expression, as an alternative plasmid

    Depositor

    Huimin Zhao

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    Codon Optimized SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEMT-3P2A

    Plasmid

    #111772

    Purpose

    Quadcistronic construct: Co-express four genes by 3 P2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p2lox-cAMPr Y205A

    Plasmid

    #102438

    Purpose

    cAMPr with a point mutation Y205A which should render PKA-C catalytically dead

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    PKA-C Y205A

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ134B

    Plasmid

    #179216

    Purpose

    Golden Gate entry vector; 4th gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU3

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hRIP3 GFP wt

    Plasmid

    #41387

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (RIPK3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSCB-hDot1Lwt

    Plasmid

    #74173

    Purpose

    For packaging hDot1Lwt to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMO9075

    Plasmid

    #117481

    Purpose

    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR

    Depositor

    Judy Wall

    Article

    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1

    Insert

    Aph(3')-IIa promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aph(3')-IIa promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKTrfp

    Plasmid

    #59772

    Purpose

    pKT230 derivative; PBAD_rfp; cmR

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    rfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFH2.1_mCherry

    Plasmid

    #205520

    Purpose

    Express mCherry (without export tag)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-EFS-N22p-3XRFPnls

    Plasmid

    #75387

    Purpose

    N22p-3XRFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    N22p
    

    Use

    Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-BRIPO

    Plasmid

    #215542

    Purpose

    BRIPO (Bioluminescent Red Indicator for Potassium)

    Depositor

    Huiwang Ai

    Article

    Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

    Insert

    BRIPO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HHM-iATPSnFR1.1

    Plasmid

    #102547

    Purpose

    expresses ATP sensor in bacteria

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pP450cam

    Plasmid

    #85085

    Purpose

    Expresses P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV_psychLight1

    Plasmid

    #198739

    Purpose

    Expresses psychLight1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

    Insert

    psychLight1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPdR

    Plasmid

    #85083

    Purpose

    Expresses putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBsaBM3

    Plasmid

    #85102

    Purpose

    P450 BM3 variant (A74G/C773S/C810S)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    P450 BM3
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    A74G/C773S/C810S

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pN3-Sp3FL

    Plasmid

    #24541

    Depositor

    Guntram Suske

    Article

    Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-1

    Insert

    transcription factor Sp3 (SP3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. ePE2

    Plasmid

    #202029

    Purpose

    Prime Editing plasmid for tag insertion

    Depositor

    Pengcheng Wei

    Article

    Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

    Insert

    Cas9，
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR_Ef1a-->flagLaM4_Notch_tTA

    Plasmid

    #162237

    Purpose

    Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaM4 mCherry nanobody Notch tTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ132-tRNA

    Plasmid

    #179211

    Purpose

    Golden Gate entry vector; 2nd gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEG03

    Plasmid

    #154138

    Purpose

    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

    Depositor

    Kang Zhou

    Article

    Panda et al Biochemical Engineering Journal

    Insert

    fucO, aldA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P_gyrA

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCM271rfp

    Plasmid

    #59774

    Purpose

    pCMrfp with TrfA mutation (271R to C)

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMIN-Ri

    Plasmid

    #197784

    Purpose

    Level 2 destination vector: pMIN-Ri backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcS-p88-C1C2

    Plasmid

    #178424

    Purpose

    p88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domain

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    p88
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Use

    Tags

    his tag and sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Cas9m4

    Plasmid

    #47316

    Purpose

    Cas9 D10A+D839A+H840A+N863A

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pG1AK-sfGFP

    Plasmid

    #71739

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRplsWT

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a_CnCas12f1

    Plasmid

    #204996

    Purpose

    CnCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

    Insert

    CnCas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCBFRE-luc

    Plasmid

    #26897

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pCMV-myc-Wnk1

    Plasmid

    #38779

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only