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We narrowed to 10,449 results for: T7

Showing: 8481 - 8520 of 10449 results

  5. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

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  9. pET21-pelB-LaG-3

    Plasmid
    #172740
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-3
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172740-plasmid-map-sequence-id-340191

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  22. pcDNA3-CBX1mut-Dronpa

    Plasmid
    #138250
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Article
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Insert
    CBX1mut-Dronpa
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138250-plasmid-map-sequence-id-307434

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  23. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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  24. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid
    #153439
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153439-plasmid-map-sequence-id-306439

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  26. pMtb MCM

    Plasmid
    #138296
    Purpose
    Expression of Mtb MCM small and large subunit
    Depositor
    Ruma Banerjee
    Article
    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d
    Inserts
    mutA
    mutB
    Use
    Tags
    His tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    inserted TEV cleavage site between His-tag and m…
    Promoter
    T7
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    138296-plasmid-map-sequence-id-292603

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  28. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133016-plasmid-map-sequence-id-262984

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  29. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

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  32. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Use
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

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Showing: 8481 - 8520 of 10449 results