Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,145 results for: TIM

Clear keywords
Showing: 10601 - 10650 of 14145 results

  1. p33Fn

    Plasmid
    #158155
    Purpose
    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.
    Depositor
    Kabin Xie
    Article
    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Rice codon optimized
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158155-plasmid-map-sequence-id-321549

    Enlarge

  4. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid
    #70656
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against DDX3Y
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

    Enlarge

  16. pXWJ109As-gfp

    Plasmid
    #123143
    Purpose
    An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, ParsR
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123143-plasmid-map-sequence-id-241757

    Enlarge

  17. pET28a-HsSENP7

    Plasmid
    #71470
    Purpose
    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pfTRAP

    Plasmid
    #50048
    Purpose
    Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells
    Depositor
    Timothy Springer
    Article
    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10
    Insert
    plamodium falciparum TRAP (TRAP Synthetic)
    Tags
    6Xhis tag
    Expression
    Mammalian
    Mutation
    C55G, N132S
    Promoter
    CMV
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pCSN074

    Plasmid
    #166731
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166731-plasmid-map-sequence-id-332203

    Enlarge

  39. pCEP4-SERTG56A -myc

    Plasmid
    #107459
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    G56A
    Promoter
    CMV
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107459-plasmid-map-sequence-id-205554

    Enlarge

  43. pCSN069

    Plasmid
    #166728
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1
    Use
    CRISPR
    Tags
    Mxi1
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166728-plasmid-map-sequence-id-332209

    Enlarge

  46. pJAID5VY

    Plasmid
    #165048
    Purpose
    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165048-plasmid-map-sequence-id-327148

    Enlarge

  50. pYPQ291

    Plasmid
    #129671
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    BthCas12b
    Use
    CRISPR; Gateway compatible bthcas12b entry clone
    Expression
    Plant
    Mutation
    BthCas12b is rice codon optimized
    Available Since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129671-plasmid-map-sequence-id-255057

    Enlarge

Showing: 10601 - 10650 of 14145 results