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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

Plasmid

#141387

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro

Plasmid

#141393

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9c

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pSRE SSG

Plasmid

#127692

Purpose

Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Structural Genome

Expression

Mammalian

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Publication

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Publication

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Expression

Bacterial

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA4TO-H2B-SunTag24x_v1

Plasmid

#60911

Purpose

Encodes a histone H2B fused to the SunTag for single molecule imaging

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and DsRed2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pJFRC18-8XLexAop2-mCD8::GFP

Plasmid

#26225

Depositor

Gerald Rubin

Publication

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-myc-Atg12

Plasmid

#24923

Depositor

Toren Finkel

Publication

Lee et al J Biol Chem. 2009 Mar 6. 284(10):6322-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Atg12 (ATG12 Human)

Tags

Myc

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV-tetO-myc T58A

Plasmid

#19763

Depositor

Konrad Hochedlinger

Publication

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FU-tet-o-hNanogP8

Plasmid

#19776

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

NanogP8 (NANOGP8 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-Flag-hSox2

Plasmid

#20073

Depositor

Juan Belmonte

Publication

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hSox2 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAd MafA-I-nGFP

Plasmid

#19412

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pcDNA 3.1(-) mouse C/EBP delta

Plasmid

#12559

Depositor

Peter Johnson

Publication

McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)

Expression

Mammalian

Mutation

S2G introduced during cloning

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Publication

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSKI015

Plasmid

#11570

Depositor

Detlef Weigel

Publication

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSV Sport PPAR gamma 1

Plasmid

#8886

Depositor

Bruce Spiegelman

Publication

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PPAR gamma (Pparg Mouse)

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles
pDonor_sU6

Plasmid

#69351

Purpose

sgRNA scaffold and synthetic U6 promoter

Depositor

Andrea Ventura

Publication

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

sU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

synthetic U6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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