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pSV Sport PPAR gamma 1

Plasmid

#8886

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-hIBA1a-GFP-miR124T

Plasmid

#214147

Purpose

AAV vector to restrict GFP expression in microglia.

Depositor

Chun-Li Zhang

Article

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Has Service

AAV5

Insert

GFP

Use

AAV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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MSP2443

Plasmid

#72252

Purpose

Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHSN6A01

Plasmid

#50586

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

dCas9-VP64
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG, HA, and NLS

Mutation

dCas9 = defective in DNA cleavage; 4×minimal VP16…

Promoter

2×35Sp and AtU6-26p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Tags

GFP

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-myc T58A

Plasmid

#19763

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hSox2

Plasmid

#20073

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hSox2 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Fyn

Plasmid

#16033

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Dominant negative: K299M

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

Plasmid

#154987

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor_sU6

Plasmid

#69351

Purpose

sgRNA scaffold and synthetic U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

sU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

synthetic U6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MDM2p-Mdm2-YFP

Plasmid

#53962

Purpose

Expresses YFP tagged Mdm2 from MDM2 promoter

Depositor

Uri Alon, Galit Lahav

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

MDM2 (MDM2 Human)

Tags

EYFP

Expression

Mammalian

Promoter

MDM2

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-lox-hOCT4

Plasmid

#20728

Depositor

Rudolf Jaenisch

Article

Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pLVPT-rtTR-KRAB

Plasmid

#11777

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has Service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTP1174

Plasmid

#104162

Purpose

Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-tdTomato-WPRE

Plasmid

#51503

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

pCAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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