We narrowed to 25,666 results for: Nes;
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Plasmid#186332PurposeThis plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.DepositorInsertvirulence genes from Agrobacterium tumefaciens pTiBo542
ExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::FLuc
Plasmid#124531PurposeSMAD2 FLuc luciferase reporterDepositorInsertSMAD FLuc reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
ExpressionPlantPromoterAtUBQ10Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:FLuc
Plasmid#118063PurposeTranscriptional unit for constitutive expression of FLuc in mammalian cellsDepositorInsertCMV:FLuc:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:RedF
Plasmid#118064PurposeTranscriptional unit for constitutive expression of RedF in mammalian cellsDepositorInsertCMV:RedF:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s-IMS
Plasmid#108120PurposeMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.DepositorInsertSypHer3s-IMS
TagsratiometricYFPExpressionMammalianPromoterCMVAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10pro-nlsABACUS2-400n-NosT
Plasmid#203728PurposeBinary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into ArabidopsisDepositorInsertnlsABACUS2-400n
UseSynthetic BiologyTagsMycExpressionPlantPromoterAtUBQ10pro 1500bpAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-MKK6-EE
Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35S:Citrine-TurboID-3xHA
Plasmid#199243PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID
Tags3xHA and TurboID-3xHAExpressionPlantPromoterCaMV 35SAvailable SinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL010097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_WiChR_TS_mScarlet_ER
Plasmid#205997PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of neuronsDepositorHas ServiceAAV9InsertWobblia Inhibitory Channelrhodopsin
UseAAVTagsTS, mScarlet, ERPromoterhSynAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3alpha2_35S:P19:Tnos (GB1203)
Plasmid#68214PurposeTranscriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoterDepositorInsertP19 silencing suppressor
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa2
Plasmid#17987DepositorAvailable SinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE FLAG-IKK2-EE puro
Plasmid#216790PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activePromoterLTRAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only