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We narrowed to 13,055 results for: SHI

Showing: 10851 - 10900 of 13055 results

  1. pSBbi-Pur-Lyn-AktAR2-D

    Plasmid
    #125198
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Article
    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:
    Insert
    Lyn-AktAR2-D
    Use
    Transposon
    Tags
    Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125198-plasmid-map-sequence-id-243596

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  2. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

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  6. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

    Plasmid
    #204359
    Purpose
    AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter
    Depositor
    Masao Doi
    Article
    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008
    Insert
    miniDq-mCherry-P2A-HA-KORD
    Use
    AAV
    Tags
    HA (for KORD) and mCherry (for miniDq)
    Expression
    Mutation
    For miniDq, the third intracellular loop (ICL3) o…
    Promoter
    hSyn
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204359-plasmid-map-sequence-id-401492

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  7. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

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  9. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  11. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJEx (Jungle Express)
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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  13. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  14. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  15. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Lesley Forrester, Antonella Fidanza
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  18. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  30. pWZL-Neo-Myr-Flag-DEST

    Plasmid
    #15300
    Purpose
    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF
    Depositor
    Jean Zhao
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Flag and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15300-plasmid-map-sequence-id-190527

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  33. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

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  37. pBFC0619

    Plasmid
    #186690
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    186690-plasmid-map-sequence-id-373180

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  39. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Martin Reijns, Andrew Jackson
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  44. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  45. AAV phSyn1(S)-DreO-bGHpA

    Plasmid
    #50363
    Purpose
    Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Has Service
    AAV Retrograde and AAV5
    Insert
    DreO recombinase
    Use
    AAV
    Tags
    none
    Expression
    Mutation
    Sequence optimized for expression in mammalian ce…
    Promoter
    hSyn1
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50363-plasmid-map-sequence-id-308988

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  47. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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Showing: 10851 - 10900 of 13055 results