We narrowed to 1,876 results for: IRES-GFP

1. pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

   Plasmid

   #134864

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   emerin (EMD Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-IRES-Puro GFP-3xFLAG

   Plasmid

   #201636

   Purpose

   Over-expression of 3xFLAG-tagged GFP

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2 LRP6-APEX2-Ires-eGFP-PAC

   Plasmid

   #180142

   Purpose

   used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

   Depositor

   Edward De Robertis

   Article

   Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

   Insert

   Human LRP6 (LRP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

   Plasmid

   #134661

   Purpose

   Expresses EGFP-tagged synaptic vesicle protein

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

   Plasmid

   #69534

   Purpose

   DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   DD-dCas9VP192-T2A-EGFP
   

   Use

   Tags

   C-term T2A-EGFP and DHFR Destabilised Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

   Plasmid

   #122966

   Purpose

   Expresses 3XFlag-tagged human CEP164 in mammalian cells

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Insert

   CEP164 (CEP164 Human)
   

   Use

   Lentiviral

   Tags

   3XFlag

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-human CSF-1Rwt-IRES-GFP

   Plasmid

   #115523

   Purpose

   Mammalian expression construct

   Depositor

   Martine Roussel

   Article

   Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

   Insert

   human CSF-1 receptor (CSF1R Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

   Plasmid

   #134865

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Membrin (GOSR2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

   Plasmid

   #126552

   Purpose

   Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

   Depositor

   Jonathan Karn

   Article

   Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

   Insert

   HIV-1 vector pNL4-3
   

   Use

   Lentiviral

   Tags

   CD8a and GFP

   Expression

   Mutation

   Delta gag/pol

   Promoter

   HIV LTR

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

    Plasmid

    #213117

    Purpose

    Expression of a faster-running MinE mutant in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE(L4E)-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L4E

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    UBC

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

    Plasmid

    #58959

    Purpose

    lentiviral expression of v-H-Ras and luciferase/EGFP reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer/chicken beta actin promoter
    v-H-Ras
    luc2/EGFP fusion gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/chicken beta actin

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

    Plasmid

    #134869

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome b5 type A (CYB5A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-98

    Promoter

    EF1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-IRES-GFP/CALR del52-FLAG

    Plasmid

    #204523

    Purpose

    Retroviral expression of human CALR del52-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR del52-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    del52

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. UbC-TRE-mSOD2-tTS-IRES-EGFP

    Plasmid

    #12390

    Depositor

    Zuoshang Xu

    Article

    Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

    Insert

    mSOD2 shRNA (Sod2 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid

    #102885

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1a-GFP-SFPQ-IRES-mCherry

    Plasmid

    #166950

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

    Plasmid

    #204497

    Purpose

    Expresses rtTA and Neomycin resistance genes in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Insert

    rtTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRRL CAG NFlag BAF170 ires GFP

    Plasmid

    #24562

    Depositor

    Jerry Crabtree

    Article

    Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

    Insert

    BAF170 (Smarcc2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pMSCV-IRES-GFP/CALR ins5-FLAG

    Plasmid

    #204524

    Purpose

    Retroviral expression of human CALR ins5-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR ins5-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    ins5

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and hSyn

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid

    #32522

    Depositor

    Mustafa Sahin

    Insert

    dominant negative Rheb (D60K) (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D60K to make dominant negative

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

24. MSCV-BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75114

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

    Plasmid

    #208695

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

    Plasmid

    #81023

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

27. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TRE-Gluc-RMA-IRES-EGFP

    Plasmid

    #189628

    Purpose

    Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and TRE

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #81022

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

30. MSCV-mouse Ezh2 Y637S-IRES-GFP

    Plasmid

    #91887

    Purpose

    Retroviral expression of mouse Ezh2 with Y637S

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Y637S

    Promoter

    MMLV gag

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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