We narrowed to 6,686 results for: tac

1. pGEX-parkin 219-465

   Plasmid

   #45972

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin 219-465 (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Dele…

   Promoter

   tac

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX6P1 UBXN11

   Plasmid

   #169017

   Purpose

   Expresses GST-UBXN11 (human) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   UBXN11 (UBXN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX2T-p19Ink4d

   Plasmid

   #52222

   Purpose

   bacterial expression of p19Ink4d

   Depositor

   Martine Roussel

   Article

   Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

   Insert

   Ink4d (Cdkn2d Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Y771F EphA2 pcDNA3

   Plasmid

   #102735

   Purpose

   Expression of EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

   Insert

   EphA2 (Epha2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y771F (TAC > TTC)

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Y593E EphA2 pcDNA3

   Plasmid

   #102731

   Purpose

   Expression of EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

   Insert

   EphA2 (Epha2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y593E (TAC > GAG)

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST-Rab8a-TN

   Plasmid

   #86080

   Purpose

   A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed threonine 22 to aspargine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-parkin W403A-C431S

   Plasmid

   #45977

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin W403A-C431S (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Doub…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMAL-c2X-ORF24-NTD

   Plasmid

   #138465

   Purpose

   Expresses MBP-tagged ORF24-NTD in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Amino acids 1-201

   Promoter

   tac

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX6P1‐Afu‐rnhBΔPIP

   Plasmid

   #108696

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C-terminal truncation (7aa) removing PIP box

   Promoter

   tac

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T1-ORF24-NTD

    Plasmid

    #138464

    Purpose

    Expresses GST-tagged ORF24-NTD in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 1-201

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST-SF3B2 wild type aa 401-550 fragment

    Plasmid

    #67615

    Purpose

    bacterial expression of wild type GST-SF3B2 fragment (401-550)

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    splicing factor 3b subunit 2 (SF3B2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment of amino acids 401-550

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Intersectin I SH3 E domain (human)

    Plasmid

    #47417

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    ITSN1 intersectin 1 (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain E

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. RBMS3_2xRRM_pGEX

    Plasmid

    #135142

    Purpose

    Recombinant expression of RBMS3 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBMS3 (RBMS3 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RBM24_RRM_pGEX

    Plasmid

    #135148

    Purpose

    Recombinant expression of RBM24 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM24 (RBM24 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GST-SF3B2 R508K aa 401-550 fragment

    Plasmid

    #79674

    Purpose

    bacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    splicing factor 3b subunit 2 (SF3B2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 451-550 with R508K

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-4T-1 dSesn (1187)

    Plasmid

    #66919

    Purpose

    bacterial expression of dSesn

    Depositor

    Michael Karin, Jun Hee Lee

    Article

    Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

    Insert

    Sesn (Sesn Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6P1‐Afu rnhB

    Plasmid

    #108694

    Purpose

    For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Insert

    rnhB (AF_RS03165 Archaeoglobus fulgidus)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-PC-2-CTS

    Plasmid

    #86068

    Purpose

    A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Polycystin-2 (Pkd2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 1-15

    Promoter

    tac

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX4T-1_OCT4_T234E_S235E

    Plasmid

    #40635

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    T234E_S235E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pGEX4T-1_OCT4_T234E

    Plasmid

    #40636

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    T234E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pGEX4T-1_OCT4_T234A

    Plasmid

    #40637

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV cleavage, and Thrombin cleavage

    Expression

    Bacterial

    Mutation

    T234A

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pGEX4T-1_OCT4_S235E

    Plasmid

    #40638

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    S235E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pGEX4T-1_OCT4_S235A

    Plasmid

    #40639

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

    Expression

    Bacterial

    Mutation

    S235A

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

24. polySUMO1

    Plasmid

    #221066

    Purpose

    Expression of polySUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMAL-MBP-Cdc42-T17N

    Plasmid

    #214289

    Purpose

    Express dominant negative Cdc42 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative Cdc42 (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Cdc42-T17N

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMAL-MBP-FRB-Cdc42/N12

    Plasmid

    #214290

    Purpose

    Express split-Cdc42 fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Cdc42 fragment fused with FRB (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMAL-MBP-Rac1-Q61L

    Plasmid

    #214292

    Purpose

    Express constitutively active Rac1 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active Rac1 (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Rac1-Q61L

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMAL-MBP-Rac1-T17N

    Plasmid

    #214293

    Purpose

    Express dominant negative Rac1 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative Rac1 (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Rac1-T17N

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMAL-MBP-FRB-Rac1/N12

    Plasmid

    #214294

    Purpose

    Express split-Rac1 fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Rac1 fragment fused with FRB (RAC1 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMAL-MBP-RhoA-Q63L

    Plasmid

    #214296

    Purpose

    Express constitutively active RhoA fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active RhoA (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    RhoA-Q63L

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMAL-MBP-RhoA-T19N

    Plasmid

    #214297

    Purpose

    Express dominant negative RhoA fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative RhoA (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    RhoA-T19N

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMAL-MBP-FRB-RhoA/N12

    Plasmid

    #214298

    Purpose

    Express split-RhoA fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-RhoA fragment fused with FRB (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX-4T1-GST-TEV-NAP1

    Plasmid

    #217610

    Purpose

    For expression in E. coli of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    TAC promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-2T_RV-B14_VP1

    Plasmid

    #202074

    Purpose

    Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    RV-B14 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX-2T_RV-A89_VP1

    Plasmid

    #202073

    Purpose

    Bacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    RV-A89 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GST-RAPH1-F2

    Plasmid

    #194864

    Purpose

    Express the RAPH1 fragment (535-868) tagged to GST in bacteria.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 1062-1250 (RAPH1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1062-1250

    Promoter

    Tac

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-6P-1 Drosophila melanogaster CBS-pair domain

    Plasmid

    #166436

    Purpose

    Express pGEX-6P-1 Drosophila melanogaster CBS-pair domain

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Drosophila melanogaster CBS-pair domain (Cbs Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac, LacI, Amp

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FKBP-EGFP-RanGAP*

    Plasmid

    #175238

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-PP-NEDD4L-WW1(193-226)

    Plasmid

    #166701

    Purpose

    expresses NEDD4L WW1 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW1 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-PP-NEDD4L-WW2(385-418)

    Plasmid

    #166702

    Purpose

    expresses NEDD4L WW2 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW2 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGEX-PP-NEDD4L-WW3(497-530)

    Plasmid

    #166703

    Purpose

    expresses NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW3 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX-PP-NEDD4L-WW4(548-581)

    Plasmid

    #166704

    Purpose

    expresses NEDD4L WW4 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L WW4 domain
    

    Use

    Tags

    GST, HRV 3C site, and Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. EcNGsC-MutY-pKK223

    Plasmid

    #131376

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFM101

    Plasmid

    #40147

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    gfp::par-2 wild-type RNAi resistant (par-2 Nematode)
    

    Use

    Tags

    GST and eGFP

    Expression

    Bacterial

    Mutation

    Base pairs 6-523 of par-2 were recoded to create …

    Promoter

    tac

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

46. pFM102

    Plasmid

    #40148

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)
    

    Use

    Tags

    GST and eGFP

    Expression

    Bacterial

    Mutation

    R183-5A; base pairs 6-523 of par-2 were recoded t…

    Promoter

    tac

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

47. pJK01_Rho_minprox_DsREd

    Plasmid

    #173489

    Purpose

    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Mouse Rhodopsin promoter driving DsRed reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Rhodopsin

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

    Plasmid

    #214879

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF2-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

    Plasmid

    #194249

    Purpose

    Expresses 3 non-targeting miRNAs

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRNegative
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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