162,717 results

1. Flag-APEX2-C Cloning Vector

   Plasmid

   #128146

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Flag-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGPD2

   Plasmid

   #43972

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GPD

   Available since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLminP_Luc2P_RE60

   Plasmid

   #90405

   Purpose

   STAT5 - Stat5 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SIE

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PER2:Luc Plasmid

   Plasmid

   #206875

   Purpose

   Per2 promoter luciferase reporter

   Depositor

   Michelle Farkas

   Article

   Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

   Insert

   Period 2 (Per2 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. DsRed2-Mito-7

   Plasmid

   #55838

   Purpose

   Localization: Mitochondria, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAVS1-TetOn-dCas9-KRAB

   Plasmid

   #115545

   Purpose

   Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p4947 pcDNA4c hBrd4 full-length

   Plasmid

   #14441

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (BRD4 Human)
   

   Use

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. NylCA-TS_pET28a

   Plasmid

   #215419

   Purpose

   Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCA-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWP78-PVCpnf_pvc13-HA

   Plasmid

   #198296

   Purpose

   Produces PVCs retargeted with HA tag

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-HA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-TetOn-mCherry-OAZ1_FS-YFP

    Plasmid

    #232356

    Purpose

    Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

    Depositor

    Ankur Jain

    Article

    Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

    Insert

    OAZ1 derived polyamine sensing module (OAZ1 Human)
    

    Use

    Lentiviral

    Tags

    eYFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUGW-FIRE-pHLy

    Plasmid

    #170774

    Purpose

    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    hUbC

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZW1-snoVector

    Plasmid

    #73174

    Purpose

    Nuclear expression of RNAs

    Depositor

    Ling-Ling Chen

    Article

    Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

    Inserts

    SNORD116-13 (SNORD116-13 Human)
    SNORD116-14 (SNORD116-14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-HIPK2

    Plasmid

    #23481

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    HIPK2 (HIPK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. TFORF0575

    Plasmid

    #143885

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXA11 (HOXA11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP scFv [N86/38]

    Plasmid

    #182095

    Purpose

    Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting GFP
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-LC3 (human)

    Plasmid

    #24920

    Purpose

    Mammalian expression of LC3 fused to EGFP

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAS-mCherry

    Plasmid

    #135457

    Purpose

    Mammalian mCherry reporter to read out FLARE and SPARK activation

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Use

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSRK2706

    Plasmid

    #78571

    Purpose

    Expresses untagged human rhinovirus 3C protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    human rhinovirus 3C protease (HRVBgp1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRH2521

    Plasmid

    #84380

    Purpose

    Expression of sgRNA from a imyc promoter (Pimyc)

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    sgRNA cloning site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3 RARE-RFP

    Plasmid

    #183054

    Purpose

    Fluorescent reporter for Retinoic Acid activity

    Depositor

    Chaya Kalcheim

    Article

    Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    RARE, SV40

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRH2502

    Plasmid

    #84379

    Purpose

    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    dcas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate at position 10 changed to Alanine, Hist…

    Promoter

    uv15tetO

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSynapsin_NLS_GFP_P2A_His6_NR1.

    Plasmid

    #170457

    Purpose

    Expresses histidine tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    Hexa-histidin

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Use

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF2997

    Plasmid

    #144473

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pci-EGFP-UPF1-WT

    Plasmid

    #135994

    Purpose

    WT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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