165,214 results

1. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-EGFP-GRAM-W

   Plasmid

   #211704

   Purpose

   Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

   Plasmid

   #55645

   Purpose

   Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV5, AAV8, and AAV9

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFR-PATagRFP

   Plasmid

   #31950

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Tags

   EGF receptor (EGFR)

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-p73DD-pcDNA3

   Plasmid

   #22978

   Depositor

   William Kaelin

   Article

   Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

   Insert

   tumor protein p73 (TP73 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   truncated cDNA encodes truncated protein (aa 327-…

   Available Since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-V5-Puro

   Plasmid

   #207730

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pPL5071_TEF1*-Cre_URA3

   Plasmid

   #60930

   Purpose

   Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.3/V5-DEST-GFP

   Plasmid

   #40125

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPRi(v2)-Blast

   Plasmid

   #170068

   Purpose

   Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLM1110_LEU2

    Plasmid

    #168460

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    mRFP1
    

    Use

    Synthetic Biology; Yeast assembly vector for big-…

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p35S_GFP-GUS

    Plasmid

    #167122

    Purpose

    Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    GFP-GUS
    

    Tags

    mGFP5, GUS

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6P-1_WT RAD23B

    Plasmid

    #201457

    Purpose

    Expresses human RAD23B with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKAR2-Br512

    Plasmid

    #161875

    Purpose

    Expresses Br512 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Maranhao et al medRxiv

    Insert

    Modified DNA Polymerase I Large Fragment (Br512)
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7, tetPA

    Available Since

    Nov. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSFFV_mNG3A(1-10)

    Plasmid

    #157992

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    W148L, M150V, K153M, N194K, F204L

    Promoter

    SFFV

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18-mini-Tn7T-Gm-lux

    Plasmid

    #64963

    Purpose

    mini-Tn7 luxCDABE transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    lux operon
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. evoBE4-SpRY

    Plasmid

    #226854

    Purpose

    Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. YES1 (T348I) in pLX303

    Plasmid

    #42564

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    T348I

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

22. HIV-dual-GT

    Plasmid

    #122696

    Purpose

    Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

    Depositor

    Klaus Überla

    Article

    Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

    Inserts

    mTagBFP2
    DsRed-max
    

    Use

    Lentiviral

    Tags

    PEST domain

    Promoter

    HIV-1 LTR

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Tags

    his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBDC

    Plasmid

    #135188

    Purpose

    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    CmR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Promoter

    cat

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBS598 EF1alpha-EGFPcre

    Plasmid

    #11923

    Purpose

    Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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26. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDD143

    Plasmid

    #119880

    Purpose

    Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pBAD_dCas9sth1
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. YES1 gRNA (BRDN0001148961)

    Plasmid

    #77967

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YES1 gRNA (BRDN0001145317)

    Plasmid

    #77965

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YES1 gRNA (BRDN0001487120)

    Plasmid

    #77966

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge