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We narrowed to 11,588 results for: EMB

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Showing: 11251 - 11300 of 11588 results

  5. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Promoter
    none
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

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  6. CD84-bio-His

    Plasmid
    #51649
    Purpose
    Expresses full-length SLAM family member 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD84 (CD84 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51649-plasmid-map-sequence-id-78973

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  13. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid
    #158766
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Mutation
    Flexible linkers between the protease and msGFP a…
    Availability
    Academic Institutions and Nonprofits only
    158766-plasmid-map-sequence-id-313130

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  16. pEHE51

    Plasmid
    #250522
    Purpose
    Vector for sgRNA expression in Acinetobacter baumannii (CRISPRi). Modified version of pYDE007 (Plasmid #194152) made Golden Gate compatible (BsaI) for sgRNA oligo insertion.
    Depositor
    Ralph Isberg
    Article
    Hamami et al bioRxiv [Preprint]. 2025 Aug 4:2024.01.0
    Insert
    Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    250522-plasmid-map-sequence-id-497877

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  23. PX4

    Plasmid
    #223340
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)
    Tags
    EGFP-miniTurboID-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223340-plasmid-map-sequence-id-455821

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  24. PX3

    Plasmid
    #223339
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)
    Tags
    miniTurboID-EGFP-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223339-plasmid-map-sequence-id-455820

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  25. PX2

    Plasmid
    #223338
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)
    Tags
    EGFP-TurboID-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223338-plasmid-map-sequence-id-455587

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  26. PX1

    Plasmid
    #223337
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)
    Tags
    TurboID-EGFP-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223337-plasmid-map-sequence-id-455823

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  33. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid
    #108288
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108288-plasmid-map-sequence-id-245273

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  34. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid
    #108290
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108290-plasmid-map-sequence-id-245275

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  37. EGFP-uTEV1-MAVS-mTA2

    Plasmid
    #171000
    Purpose
    HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A, R538A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A, R538A]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171000-plasmid-map-sequence-id-335835

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  38. EGFP-uTEV1-MAVS-mTA5

    Plasmid
    #171003
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI, R537A]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171003-plasmid-map-sequence-id-335793

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  43. pBB0238-TEV-His12

    Plasmid
    #137038
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137038-plasmid-map-sequence-id-291947

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  45. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

    Plasmid
    #127403
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available Since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    127403-plasmid-map-sequence-id-248720

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  46. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

    Plasmid
    #127407
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon, K305H,…
    Promoter
    CMV
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127407-plasmid-map-sequence-id-247970

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  47. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

    Plasmid
    #127401
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127401-plasmid-map-sequence-id-247963

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  49. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

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Showing: 11251 - 11300 of 11588 results